PMID: 994Sep 1, 1975

The interaction of magnesium ions with teichoic acid

The Biochemical Journal
P A LambertJ Baddiley

Abstract

The binding of Mg2+ to the wall teichoic acid of Lactobacillus buchneri N.C.I.B. 8007 was measured by equilibrium dialysis at controlled ionic concentration and pH. In an aqueous solution containing 10mM-NaCl at pH 5.0 one Mg2+ ion was bound for every two phosphate groups of the teichoic acid, with an apparent association constant, Kassoc. = 2.7 x 10(3) M-1. On lowering the pH below the pKa of the phosphate groups the amount of bound Mg2+ decreased concomitantly with decreasing ionization of the phosphate groups. Both the amount of Mg2+ bound to the teichoic acid and the apparent association constants were similar in the presence of 10 mM concentrations of NaCl or KCl but decreased markedly in the presence of 10 mM-CaCl2 because of competition between Ca2+ and Mg2+ for the binding sites. A similar effect was found when the concentration of NaCl was increased from 0 to 50 mM. The results are discussed in relation to the function of teichoic acid in the walls of Gram-positive bacteria.

Citations

Oct 13, 2012·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·I-Chiao LeePeter A Bron
Jun 30, 2015·The Science of the Total Environment·Rama PulicharlaJose R Valero
Dec 23, 2006·International Journal of Food Microbiology·T HalttunenR Tahvonen
May 15, 2015·Biochimica Et Biophysica Acta·Kieth J Thomas, Charles V Rice
Dec 4, 2003·Biophysical Journal·Prisca Schär-Zammaretti, Job Ubbink
Jul 1, 1979·Chemico-biological Interactions·W Barrow, T G Tornabene
Oct 25, 2011·Applied and Environmental Microbiology·Anisha GoelDouwe Molenaar
Jan 1, 1987·Critical Reviews in Microbiology·R J Doyle, A L Koch
Oct 3, 2006·Journal of Bacteriology·Michael A D'EliaEric D Brown
May 5, 2006·Journal of Bacteriology·Tomaz KoprivnjakJerrold P Weiss
Aug 17, 2010·Journal of Bacteriology·Maria Karatsa-DodgsonAngelika Gründling
Dec 11, 2003·Microbiology and Molecular Biology Reviews : MMBR·Francis C Neuhaus, J Baddiley
May 11, 2018·Applied Microbiology and Biotechnology·Rin Endo, Hideki Aoyagi

Related Concepts

Calcium Chloride, Anhydrous
Cell Wall
Hydrogen-Ion Concentration
Lactobacillus
Magnesium
Slow-K
Sodium Chloride, (24)NaCl
Teichoic Acids

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