The interaction of MC3R and MC4R with MRAP2, ACTH, α-MSH and AgRP in chickens

The Journal of Endocrinology
Jiannan ZhangYajun Wang

Abstract

The interaction of melanocortin-4 (MC4R) and melanocortin-3 (MC3R) receptors with proopiomelanocortin (POMC)-derived peptides (e.g. α-MSH), agouti-related protein (AgRP) and melanocortin-2 receptor accessory protein 2 (MRAP2) is suggested to play critical roles in energy balance of vertebrates. However, evidence on their interaction in birds remains scarce. Our study aims to reveal their interaction in chickens and the results showed that (1) chicken (c-)MC3R and cMC4R expressed in Chinese hamster ovary (CHO) cells can be activated by α-MSH and ACTH1-39 equipotently, monitored by a pGL3-CRE-luciferase reporter system; (2) cMC3R and cMC4R, when co-expressed with cMRAP2 (or cMRAP, a cMRAP2 homolog), show increased sensitivity to ACTH treatment and thus likely act as ACTH-preferring receptors, and the interaction between cMC3R/cMC4R and cMRAP2 was demonstrated by co-immunoprecipitation assay; (3) both cMC3R and cMC4R display constitutive activity when expressed in CHO cells, as monitored by dual-luciferase reporter assay, and cMRAP2 (and cMRAP) can modulate their constitutive activity; (4) AgRP inhibits the constitutive activity of cMC3R/cMC4R, and it also antagonizes ACTH/α-MSH action on cMC4R/cMC3R, indicating that AgRP function...Continue Reading

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Citations

Nov 8, 2018·Journal of Molecular Endocrinology·Ming ZhuChao Zhang
Aug 17, 2019·Frontiers in Endocrinology·Nicolas HeyderPatrick Scheerer
Aug 14, 2020·International Journal of Molecular Sciences·Gunnar KleinauPatrick Scheerer
Jan 18, 2019·Journal of the Endocrine Society·Lucia SolettoJosé Miguel Cerdá-Reverter
Jul 17, 2020·Frontiers in Physiology·Charlene HanlonGrégoy Y Bédécarrats
Feb 2, 2021·Journal of Cellular Physiology·Xiaolu TaiChao Zhang
Apr 4, 2021·Biomolecules·Zheng-Yong WenYa-Xiong Tao
May 12, 2020·Molecular and Cellular Endocrinology·Ya-Xiong Tao

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