The interaction of SiO2 nanoparticles with the neuronal cell membrane: activation of ionic channels and calcium influx

Nanomedicine
C DistasiD Lovisolo

Abstract

To clarify the mechanisms of interaction between SiO2 nanoparticles (NPs) and the plasma membrane of GT1-7 neuroendocrine cells, with focus on the activation of calcium-permeable channels, responsible for the long lasting calcium influx and modulation of the electrical activity in these cells. Nontoxic doses of SiO2 NPs were administered to the cells. Calcium imaging and patch clamp techniques were combined with a pharmacological approach. TRPV4, Cx and Panx-like channels are the major components of the NP-induced inward currents. Preincubation with the antioxidant N-acetyl-L-cysteine strongly reduced the [Ca2+]i increase. These findings suggest that SiO2 NPs directly activate a complex set of calcium-permeable channels, possibly by catalyzing free radical production.

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Citations

Jun 1, 2019·Biochimica Et Biophysica Acta. Biomembranes·Daniele ZanellaGiovanni Bernardini

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Methods Mentioned

BETA
transmission electron microscopy
electrophoretic light scattering

Software Mentioned

Axoscope
Clampex
Metafluor
OriginPro
PClamp Axoscope

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