The interaction of sperm cells with exogenous DNA: a role of CD4 and major histocompatibility complex class II molecules

Experimental Cell Research
Marialuisa LavitranoC Spadafora

Abstract

Mouse epidydimal sperm cells have the spontaneous ability to take up exogenous DNA, a part of which is further internalized into nuclei. We report here that sperm cells from MHC class II knockout mice have a reduced ability to bind DNA compared to sperm cells from wild-type animals. Spermatozoa from CD4 knockout mice are instead fully capable of binding exogenous DNA, yet lose the ability to further internalize it. MHC class II expression was not detected on sperm heads using monoclonal antibodies. In contrast, CD4 molecules were found on sperm heads by both immunofluorescence and Western blot analysis. Moreover, we show that nuclear internalization of exogenous DNA was prevented in wild-type sperm cells preincubated with anti-CD4 mAbs. These results support the conclusion that CD4 and MHC class II molecules play distinct roles in the process of sperm/DNA interaction: though not present in mature sperm cells, MHC class II expression appears to be required during spermatogenesis to produce sperm cells capable of taking up foreign DNA, while CD4 molecules present on sperm cells mediate the nuclear internalization of sperm-bound DNA.

References

Mar 1, 1991·The Journal of Experimental Medicine·A Pelchen-MatthewsM Marsh
Sep 6, 1991·Cell·D CosgroveD Mathis
Jul 23, 1987·Nature·B P SleckmanS J Burakoff
Feb 11, 1982·Nature·V L ScofieldI L Weissman
Jul 7, 1995·Science·R J Aitken
Nov 1, 1994·The Journal of Cell Biology·B BaccettiA Massacesi

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Citations

Jul 29, 2010·Zygote : the Biology of Gametes and Early Embryos·Qiuyan LiXiao-Rong An
Feb 2, 2000·Animal Biotechnology·K R Smith
May 31, 2001·Microbiology and Molecular Biology Reviews : MMBR·N Dejucq, B Jégou
Oct 16, 1999·Proceedings of the National Academy of Sciences of the United States of America·M S ChenJ M White
Oct 24, 2002·Proceedings of the National Academy of Sciences of the United States of America·Marialuisa LavitranoGiancarlo Rossi
Sep 14, 2007·Archives of Andrology·Kevin CowardJohn Parrington
Jan 13, 2011·Systems Biology in Reproductive Medicine·John ParringtonJoaquin Gadea
Jan 25, 2016·Theriogenology·Paulo Varoni CavalcantiMayra Elena Ortiz D'Avila Assumpção
Jun 15, 2011·Animal Reproduction Science·Vinicius F CamposTiago Collares
Jun 12, 2010·Theriogenology·W B FeitosaM E O A Assumpção
Dec 24, 2008·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Yidong Niu, Shulong Liang
May 8, 2007·Biochemical and Biophysical Research Communications·Shu-Yan YangWei-Quan Liu
May 21, 2005·Molecular Reproduction and Development·Nicole L WebsterMarialuisa Lavitrano
Apr 16, 2005·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Kevin Smith, Corrado Spadafora
Jan 28, 2003·Molecular Reproduction and Development·Marialuisa LavitranoEraldo Seren
Nov 25, 2003·American Journal of Reproductive Immunology : AJRI·W W C Chiu, L W Chamley
Oct 1, 2009·Reproduction in Domestic Animals = Zuchthygiene·T S KimD S Lee
Aug 2, 2005·Theriogenology·M Anzar, M M Buhr
Jan 5, 2001·Experimental Cell Research·R Carballada, P Esponda
Jun 18, 2011·Forensic Science International. Genetics·Joana PereiraKlaus Olek
Mar 5, 2016·The Journal of Reproduction and Development·Shahram ChaparianShahin Eghbalsaied
May 29, 2000·Molecular Reproduction and Development·P J Chan
Dec 25, 2002·Molecular Therapy : the Journal of the American Society of Gene Therapy·Jose B Cibelli
Oct 9, 2009·Biology of Reproduction·Chong LiTeruhiko Wakayama
Feb 16, 2006·Reproduction, Fertility, and Development·Marialuisa LavitranoAlessia Vargiolu
Jul 14, 2017·Zygote : the Biology of Gametes and Early Embryos·William Borges DominguesVinicius Farias Campos
Sep 14, 2018·Zygote : the Biology of Gametes and Early Embryos·Zigomar da SilvaMariana Groke Marques
Nov 1, 2018·Zygote : the Biology of Gametes and Early Embryos·Soleiman KurdMohammad Salehi
Jul 11, 2006·Biochemistry. Biokhimii︠a︡·B P ChelobanovV V Vlasov
Apr 24, 2010·Journal of Biosciences·Tiago CollaresJoao Carlos Deschamps
Oct 19, 2011·International Journal of Legal Medicine·Andrew John Hopwood, Keith Elliott
Aug 29, 2019·Proceedings. Biological Sciences·Ilaria SciamannaCorrado Spadafora
Oct 17, 2012·Molecular Reproduction and Development·Sergio OddiMauro Maccarrone
Mar 29, 2000·Theriogenology·F Gandolfi

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