The interaction of taurine-salicylaldehyde Schiff base copper(II) complex with DNA and the determination of DNA using the complex as a fluorescence probe

Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
Xiaoyan ZhangZhousheng Yang

Abstract

The interaction of taurine-salicylaldehyde Schiff base copper(II) (Cu(TSSB)2(2+)) complex with DNA was explored by using UV-vis, fluorescence spectrophotometry, and voltammetry. In pH 7.4 Tris-HCl buffer solution, the binding constant of the Cu(TSSB)2(2+) complex interaction with DNA was 3.49 x 10(4) L mol(-1). Moreover, due to the fluorescence enhancing of Cu(TSSB)2(2+) complex in the presence of DNA, a method for determination of DNA with Cu(TSSB)2(2+) complex as a fluorescence probe was developed. The fluorescence spectra indicated that the maximum excitation and emission wavelength were 389 nm and 512 nm, respectively. Under optimal conditions, the calibration graphs are linear over the range of 0.03-9.03 microg mL(-1) for calf thymus DNA (CT-DNA), 0.10-36 microg mL(-1) for yeast DNA and 0.01-10.01 microg mL(-1) for salmon DNA (SM-DNA), respectively. The corresponding detection limits are 7 ng mL(-1) for CT-DNA, 3 ng mL(-1) for yeast DNA and 3 ng mL(-1) for SM-DNA. Using this method, DNA in synthetic samples was determined with satisfactory results.

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Citations

Apr 3, 2014·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Ran-ran DuanXiao-hua Zhou
May 4, 2013·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Shuyun BiLili Yan
Feb 5, 2013·Medical Hypotheses·Jiang-Ning WangDe-Cheng Wang
Jun 19, 2013·International Journal of Biological Macromolecules·Shaoguang GengFengling Cui
Nov 30, 2011·Dalton Transactions : an International Journal of Inorganic Chemistry·Zhijun ZhangWenjing Xu
May 24, 2014·Journal of Biomolecular Structure & Dynamics·Jagadeesan LakshmiprabaMohammad Abdulkader Akbarsha
Jul 21, 2016·International Journal of Biological Macromolecules·Sinara Mônica Vitalino de AlmeidaMaria do Carmo Alves de Lima
Dec 20, 2013·Journal of the Royal Society, Interface·Francesca FassioliGregory D Scholes

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