PMID: 7541281Apr 1, 1995Paper

The interaction of trichloroethanol with murine recombinant 5-HT3 receptors

British Journal of Pharmacology
D L DownieN M Barnes

Abstract

1. The effects of ethanol, chloral hydrate and trichloroethanol upon the 5-HT3 receptor have been investigated by use of electrophysiological techniques applied to recombinant 5-HT3 receptor subunits (5-HT3R-A or 5-HT3R-As) expressed in Xenopus laevis oocytes. Additionally, the influence of trichloroethanol upon the specific binding of [3H]-granisetron to membrane preparations of HEK 293 cells stably transfected with the murine 5-HT3R-As subunit and 5-HT3 receptors endogenous to NG 108-15 cell membranes was assessed. 2. Ethanol (30-300 mM), chloral hydrate (1-30 mM) and trichloroethanol (0.3-10 mM), produced a reversible, concentration-dependent, enhancement of 5-HT-mediated currents recorded from oocytes expressing either the 5-HT3R-A, or the 5-HT3R-As subunit. 3. Trichloroethanol (5 mM) produced a parallel leftward shift of the 5-HT concentration-response curve, reducing the EC50 for 5-HT from 1 +/- 0.04 microM (n = 4) to 0.5 +/- 0.01 microM (n = 4) for oocytes expressing the 5-HT3R-A. A similar shift, from 2.1 +/- 0.05 microM (n = 11) to 1.3 +/- 0.1 microM (n = 4), was observed in oocytes expressing the 5-HT3R-As subunit. Trichloroethanol (5 mM) had little or no effect upon the maximum current produced by 5-HT for either rec...Continue Reading

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Citations

Sep 16, 1999·Cellular and Molecular Life Sciences : CMLS·M D Krasowski, N L Harrison
Mar 1, 1996·Trends in Pharmacological Sciences·R M ParkerN M Barnes
Mar 11, 2000·Brain Research·S M O'SheaN L Harrison
Jul 8, 2000·European Journal of Pharmacology·S MochizukiK Furuichi
Jul 16, 1999·Neurochemistry International·D M Lovinger
Feb 27, 1999·Toxicology Letters·D M Lovinger, Q Zhou
Apr 1, 1996·British Journal of Pharmacology·A JenkinsW R Lieb
Mar 5, 2010·The Journal of Pharmacology and Experimental Therapeutics·Paula L Feinberg-Zadek, Paul A Davies
Aug 26, 1999·Neuropharmacology·N M Barnes, T Sharp
Feb 6, 2013·Trends in Pharmacological Sciences·Andrew J Thompson
Jul 5, 2013·British Journal of Pharmacology·A J Thompson, S C R Lummis
Mar 16, 2013·British Journal of Pharmacology·A J Thompson, S C R Lummis
Apr 19, 2013·British Journal of Pharmacology·Amy S NewmanNicholas M Barnes
May 10, 2005·European Journal of Pharmacology·Gábor MaksayGyula Bugovics
Nov 3, 2016·British Journal of Pharmacology·Andrew D PowellNicholas M Barnes
Sep 10, 1998·Molecular Endocrinology·C H WetzelR Rupprecht
May 17, 2003·The Journal of Biological Chemistry·Gary W BoydChristopher N Connolly

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