PMID: 9179432May 1, 1997Paper

The interaction of tumour necrosis factor alpha and endothelin-1 in pathogenetic models of asthma

Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology
J Xu, N S Zhong

Abstract

There is evidence that tumour necrosis factor alpha (TNF alpha) may be an important mediator in initiating asthmatic airway inflammation. It has been proposed that endothelin-1 (ET-1) is involved in bronchoconstriction and airway remodelling in asthma. It is not known, however, if there is any interaction between TNF alpha and ET in perpetuating airway inflammation in asthma. The present study aimed to determine the activities of ET-1 and TNF alpha in ovalbumin-sensitized guinea pigs and their roles in the development of airway inflammation. Twelve guinea pigs were sensitized by ovalbumin injection and aerosol inhalation. ET-1 levels were measured in both bronchoalveolar lavage fluid (BALF) and plasma by 125-labelled endothelin-1 (ET-1) radioimmunoassay. The TNF alpha activity released from alveolar macrophage (AM) in BALF was estimated by ELISA. Cultured bovine airway smooth muscle cells (BASMCs) were treated with TNF alpha (1000 units/5 x 10(4) cells) for different times. ET-1 levels in harvested medium from these cells were measured by radioimmunoassay. Cultured human fetal lung fibroblasts (HFLFs) were incubated with ET-1 (10(-8) approximately 10(-6)M), then 3HTdR incorporation to these cells and cell counting were performe...Continue Reading

Citations

Dec 28, 1999·Respirology : Official Journal of the Asian Pacific Society of Respirology·J Xu, N S Zhong
Jan 16, 2009·Respirology : Official Journal of the Asian Pacific Society of Respirology·Li LiJian Kang
Nov 2, 2007·Respiratory Research·Ziemowit ZietkowskiAnna Bodzenta-Lukaszyk
May 2, 2007·BMC Pulmonary Medicine·Camille TailléBruno Crestani
Dec 17, 2008·American Journal of Physiology. Heart and Circulatory Physiology·Fernanda R C GiachiniRita C Tostes
Feb 28, 2004·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·T-N WangY-C Ko
Dec 19, 2003·Human Molecular Genetics·Hyoung Doo ShinChoon-Sik Park

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