PMID: 7538054Feb 16, 1995Paper

The interaction of vascular endothelial cells and dorsal root ganglion neurites is mediated by vitronectin and heparan sulfate proteoglycans

Brain Research. Developmental Brain Research
K Isahara, M Yamamoto

Abstract

The interaction of peripheral nerve and blood vessels during development was studied by using DRG explant culture plated on confluent monolayer of vascular endothelial cells (VEC). The comparison of neurite length on various substrates showed a preference of DRG neurites in the following order; thrombospondin > laminin, vitronectin > fibronectin, VEC monolayer > collagen I, rat astrocyte monolayer. On layers of fibroblasts (3T3) or gliomas (C6), neurite extension was not observed. To identify the neurite outgrowth promoting adhesion molecules on VEC surface, several antibodies and synthetic peptides were added to the culture medium of DRG. With vitronectin antibody or with peptides containing the Arg-Gly-Asp (RGD) sequence, 30-40% of neurite outgrowth was inhibited and these two effects were not additive. Therefore, a part of neurite outgrowth in this system is mediated by vitronectin in RGD dependent manner. Another molecule which promotes neurite outgrowth on VEC was identified by a new monoclonal antibody (MAb) EC1. In the Western blot analysis, the immunoreactive band which was over 400 kDa was intensified by guanidine HCl extraction. EC1 immunoreactive band disappeared after the treatment of heparitinase but not with other...Continue Reading

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Citations

Nov 7, 2013·BioMed Research International·Stefan Plantman
Jul 2, 2008·Molecular and Cellular Neurosciences·Stefan PlantmanStaffan Cullheim
Oct 20, 2018·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Papon MuangsanitJames B Phillips
May 7, 2008·Biomedical Materials·Patricia Musoke-Zawedde, Molly S Shoichet
Jul 5, 2001·The Journal of Comparative Neurology·K Y ChungS O Chan
Feb 23, 2000·Experimental Neurology·L D MoonJ W Fawcett

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