PMID: 7032516Jul 1, 1981Paper

The interaction of yeast hexokinase with Procion Green H-4G

The Biochemical Journal
Y D ClonisC R Lowe

Abstract

1. A number of reactive triazine dyes specifically and irreversibly inactive yeast hexokinase at pH 8.5 and 33 degrees C. Under these conditions, the enzyme is readily inactivated by 100 microM-Procion Green H-4G, Blue H-B, Turquoise H-7G and Turquoise H-A, is less readily inactivated by Procion Brown H-2G. Green HE-4BD, Red HE-3B and Yellow H-5G and is not inactivated at all by Procion Yellow H-A. 2. The inactivation of hexokinase by Procion Green H-4G is competitively inhibited by the adenine nucleotides ATP and ADP and the sugar substrates D-glucose, D-mannose and D-fructose but not by nonsubstrates such as D-arabinose and D-galactose. 3. Quantitatively inhibited hexokinase contains approx. 1 mol of dye per mol of monomer of mol.wt. 51000. The inhibition is irreversible and activity cannot be recovered on incubation with high concentration (20 mM) of ATP or D-glucose. 4. Mg2+ protects the enzyme against inactivation by Procion Green H-4G but enhances the rate of inactivation by all the other Procion dyes tested. In the presence of 10 mM-Mg2+ the apparent dissociation constant between enzyme and dye is reduced from 199.0 microM to 41.6 microM. Binding of the dye to hexokinase is accompanied by characteristic spectral changes ...Continue Reading

Citations

Oct 13, 2000·Journal of Enzyme Inhibition·N E Labrou
Jan 1, 1988·Critical Reviews in Biotechnology·Y D Clonis
Jul 1, 1982·European Journal of Biochemistry·J E McArdellC J Bruton
Nov 9, 2002·European Journal of Biochemistry·C F MazitsosY D Clonis
Oct 5, 1987·Biotechnology and Bioengineering·Y D ClonisC R Lowe
Dec 5, 1992·Biotechnology and Bioengineering·A G MayesJ Hubble
Oct 1, 1987·Applied Biochemistry and Biotechnology·G VlatakisY D Clonis
Jun 1, 2011·Applied Microbiology and Biotechnology·Baokun LiWei Chen
Apr 11, 1986·Journal of Chromatography·C R LoweV Stead
Jan 4, 1982·Biochimica Et Biophysica Acta·P HughesR F Sherwood
Aug 15, 1994·Journal of Biotechnology·N Labrou, Y D Clonis
May 1, 1988·Journal of Biochemical and Biophysical Methods·L MiribelP Arnaud
Aug 1, 1987·Journal of Biochemical and Biophysical Methods·L Miribel, P Arnaud
Oct 26, 2005·Journal of Chromatography. a·Yannis D Clonis

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