The interactions between cysteamine, cystine and cumulus cells increase the intracellular glutathione level and developmental capacity of goat cumulus-denuded oocytes

Reproduction : the Official Journal of the Society for the Study of Fertility
Ping ZhouJing-He Tan

Abstract

To improve in vitro maturation (IVM) of denuded oocytes (DOs), we observed the interactive effects of cysteamine, cystine and cumulus cells on the glutathione (L-gamma-glutamyl-L-cysteinyl-glycine; GSH) level and developmental capacity of goat IVM oocytes. Cysteamine supplementation increased the GSH level and blastocyst rates of both cumulus-oocyte complexes (COCs) and DOs, while the addition of cystine increased the GSH level and blastulation only in the presence of cumulus cells (COCs or DOs co-cultured on a cumulus cell monolayer). Simultaneous supplementation of cysteamine and cystine increased the GSH content and blastulation of co-cultured DOs to a level similar to that of COCs matured without thiol supplementation. Co-culture without thiol supplementation improved DOs' GSH synthesis but not blastulation. The results suggest that DOs cannot utilize cystine for GSH synthesis unless exogenous cysteamine is supplied by either cumulus cells or supplementation. Thus, while the addition of cystine alone is enough to improve IVM of COCs, improvement of DOs requires supplementation of both cystine and cysteamine. Synergic actions between cysteamine, cystine and cumulus cells restore the GSH level and developmental capacity of go...Continue Reading

References

Jan 1, 1976·Annual Review of Biochemistry·A Meister, S S Tate
Jul 1, 1979·Physiological Reviews·B ChanceA Boveris
Sep 3, 1984·Biochimica Et Biophysica Acta·S Bannai
Apr 1, 1984·Developmental Biology·A C Schroeder, J J Eppig
Apr 29, 1983·Science·A Meister
Mar 1, 1995·Molecular Reproduction and Development·L ZhangR A Godke
Dec 1, 1995·Molecular Reproduction and Development·D G de MatosH Baldassarre
Nov 24, 1999·Free Radical Biology & Medicine·A P Arrigo
Feb 24, 2001·Theriogenology·B GasparriniL Zicarelli
Feb 9, 2002·Molecular Reproduction and Development·Sofie TangheAart de Kruif
Dec 31, 2002·Molecular Reproduction and Development·D G de MatosJ Smitz
Apr 12, 2005·Reproduction in Domestic Animals = Zuchthygiene·P WongsrikeaoT Otoi
Apr 19, 2007·Molecular Reproduction and Development·Li GeJing-He Tan

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Citations

Apr 1, 2010·Reproduction, Fertility, and Development·E C CurnowE S Hayes
Dec 21, 2013·Journal of Assisted Reproduction and Genetics·Amir Hossein Abazari-KiaMohammad Salehi
Sep 18, 2013·Reproduction : the Official Journal of the Society for the Study of Fertility·Hua-Yu LianJing-He Tan
Mar 22, 2014·Theriogenology·Joanna Maria Gonçalves de Souza-FabjanPascal Mermillod
Aug 5, 2014·The Journal of Reproduction and Development·Robert E Click
Jul 11, 2009·Fertility and Sterility·Jin-Hua LuJing-He Tan
Mar 10, 2010·Reproduction in Domestic Animals = Zuchthygiene·S Deleuze, G Goudet
Aug 13, 2015·The Journal of Endocrinology·Yan-Guang WuNorbert Gleicher
Mar 24, 2018·Reproduction, Fertility, and Development·Yun-Gwi ParkSe-Pill Park
Feb 16, 2019·Zygote : the Biology of Gametes and Early Embryos·A A A SilvaE S Lopes Júnior
Dec 26, 2018·Journal of Pineal Research·Linghua ChengLei An
Aug 11, 2020·Zygote : the Biology of Gametes and Early Embryos·Seungbum HongNisar Ahmad Wani

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