The intercellular communication via nitric oxide and its regulation in coupling of cyclic GMP synthesis upon stimulation of muscarinic cholinergic receptors in rat superior cervical sympathetic ganglia

Brain Research
M AndoY Nagata

Abstract

Cyclic GMP (cGMP) production in rat superior cervical sympathetic ganglia (SCG) was markedly increased (ca. 7-9-fold) by the addition of either acetylcholine (ACh; 0.1 mM) or a muscarinic agonist, carbachol (Carb; 0.1 mM), in the presence of an inhibitor (3-isobutyl-1-methylxanthine) for cGMP hydrolytic enzyme during in vitro aerobic incubation at 37 degrees C for 5 min. The ACh-induced accumulation of cGMP in SCG was effectively blocked (-73%) by the further addition of atropine (10 microM), a muscarinic antagonist, whereas a nicotinic blocker, hexamethonium (10 microM) partially antagonized (-41%) this ACh stimulation. The inhibitory effect of hexamethonium on ACh-evoked ganglionic cGMP production was effectively augmented (-83%) by addition of NG-monomethyl-L-arginine (L-NMMA, 50 microM), a compound that inhibits nitric oxide (NO) synthesis from L-arginine. Comparable inhibition of cGMP formation was observed following application of L-NMMA to the SCG upon stimulation of Carb. In contrast, L-NMMA had no effect on the decreased level of ACh-evoked cGMP production caused by the muscarinic antagonist. The Carb-induced elevation of ganglionic cGMP synthesis was significantly reduced within 1 min of incubation in the medium conta...Continue Reading

References

Mar 24, 1992·Biochemistry·T G Traylor, V S Sharma
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Apr 1, 1991·Trends in Pharmacological Sciences·S H Snyder, D S Bredt
Jul 1, 1988·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·E E el-FakahanyJ M Baraban
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Citations

Oct 19, 2019·The European Journal of Neuroscience·Nilson Carlos Ferreira-JuniorKarla Nívea Sampaio
Aug 14, 1999·Journal of Biological Rhythms·I V ZhdanovaJ A Taylor

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