The interdigitated beta-helix domain of the P22 tailspike protein acts as a molecular clamp in trimer stabilization.

Protein Science : a Publication of the Protein Society
J F KreisbergJonathan King

Abstract

The P22 tailspike adhesin is an elongated thermostable trimer resistant to protease digestion and to denaturation in sodium dodecyl sulfate. Monomeric, dimeric, and protrimeric folding and assembly intermediates lack this stability and are thermolabile. In the native trimer, three right-handed parallel beta-helices (residues 143-540), pack side-by-side around the three-fold axis. After residue 540, these single chain beta-helices terminate and residues 541-567 of the three polypeptide chains wrap around each other to form a three-stranded interdigitated beta-helix. Three mutants located in this region -- G546D, R563Q, and A575T -- blocked formation of native tailspike trimers, and accumulated soluble forms of the mutant polypeptide chains within cells. The substitutions R563Q and A575T appeared to prevent stable association of partially folded monomers. G546D, in the interdigitated region of the chain, blocked tailspike folding at the transition from the partially-folded protrimer to the native trimer. The protrimer-like species accumulating in the G546D mutant melted out at 42 degrees C and was trypsin and SDS sensitive. The G546D defect was not corrected by introduction of global suppressor mutations, which correct kinetic de...Continue Reading

References

Jul 2, 1991·Biochemistry·A FuchsR Seckler
Jan 1, 1991·Annual Review of Biophysics and Biophysical Chemistry·J Engel, D J Prockop
Dec 28, 1971·Journal of Molecular Biology·J King, U K Laemmli
Dec 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·D P GoldenbergJ King
Nov 9, 1982·Biochemistry·R T SauerP B Berget
Jun 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·D Goldenberg, J King
May 1, 1995·Protein Science : a Publication of the Protein Society·M A SpeedJ King
Nov 1, 1993·Protein Science : a Publication of the Protein Society·M Danner, R Seckler
Jan 1, 1997·Protein Science : a Publication of the Protein Society·M A SpeedJ King
Jul 31, 1998·Protein Science : a Publication of the Protein Society·S D Betts, J King
Feb 27, 1999·Current Opinion in Structural Biology·J Baum, B Brodsky
Feb 24, 2001·Protein Science : a Publication of the Protein Society·J F KreisbergJ King
Mar 27, 2001·Protein Science : a Publication of the Protein Society·C Haase-PettingellJ King

❮ Previous
Next ❯

Citations

Jun 4, 2010·The Journal of Biological Chemistry·James E ThompsonIan S Roberts
Aug 6, 2004·Applied and Environmental Microbiology·Welkin H PopeJonathan King
Jul 3, 2003·Journal of Bacteriology·Peter R WeigeleJonathan King
Oct 20, 2005·Journal of Bioscience and Bioengineering·Surinder Mohan Singh, Amulya Kumar Panda
Mar 1, 2006·Proceedings of the National Academy of Sciences of the United States of America·Ryan Simkovsky, Jonathan King
Jul 29, 2004·European Journal of Oral Sciences·Ljubomir VitkovWolf Dietrich Krautgartner
Nov 18, 2005·Journal of Molecular Biology·Peter R WeigeleJonathan King
Nov 23, 2006·Gene·Milka Zayas, Robert Villafane
Nov 25, 2003·Protein Science : a Publication of the Protein Society·Matthew J Gage, Anne Skaja Robinson
Aug 24, 2004·Protein Science : a Publication of the Protein Society·Scott BettsJonathan King
Aug 6, 2005·Protein Science : a Publication of the Protein Society·Matthew J GageAnne Skaja Robinson
Dec 29, 2006·The Journal of Biological Chemistry·Woo-Suk JungSangkee Rhee
Jan 18, 2017·Cold Spring Harbor Perspectives in Biology·Jose A RodriguezDavid S Eisenberg
Nov 11, 2017·Extremophiles : Life Under Extreme Conditions·Masahito MurakamiYoko Nagata
Apr 19, 2005·The Journal of Biological Chemistry·Madhulika JainPatricia L Clark

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.