The interferon-alpha genes from three chicken lines and its effects on H9N2 influenza viruses

Animal Biotechnology
Chun XiaMin Wang

Abstract

The interferon-alpha genes from three chicken lines were cloned by a direct PCR technique, and the effects of recombinant protein expressed in a prokaryotic system on highly pathogenic H9N2 influenza viruses were investigated. The cloned ChIFN-alpha gene encoded a protein of 193 amino acids with a signal sequence of 31 amino acids and mature peptides of 162 amino acids. Comparison of ChIFN-alpha sequences, detected six amino acids substitutions at positions 50, 58, 65, 81, 181, and 183. Homology analysis indicated that ChIFN-alpha genes could be subdivided into two lineages, SH-ChIFN-alpha and WJ-ChIFN-alpha. In addition, both SH-ChIFN-alpha and WJ-ChIFN-alpha were expressed with the N-terminal 6 consecutive histidine residues in a high-level prokaryotic expression system. Recombinant chicken interferon-alpha (rChIFN-alpha) protein has anti-VSV activity of more than 1 x 10(8) U/mg. Moreover, High concentration (10,000U) of rSH-ChIFN-alpha resulted in over 40% inhibition of the H9N2 virus infection in chicken embryos (Ovo), and 100% inhibition from one day-old to five day-old chickens (Vivo). The results suggested that rChIFN-alpha is a potential agent against many Chicken viral strains.

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Citations

Apr 20, 2006·Animal Biotechnology·Xiju ShiMing Wang
May 18, 2006·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Li WangChun Xia
Feb 18, 2011·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Shanshan MengWenjun Liu
Apr 25, 2008·Viral Immunology·Mohammad HeidariShayan Sharif
May 27, 2008·Journal of Zhejiang University. Science. B·Li SongYi Li
Oct 25, 2011·Avian Diseases·Ming XuMohammad Heidari
Feb 9, 2008·Emerging Infectious Diseases·Philip I MarcusMargaret J Sekellick
Nov 29, 2016·The Journal of Veterinary Medical Science·Jun ZhaoJason Chen
Feb 16, 2017·Frontiers in Immunology·Diwakar SanthakumarMuhammad Munir
Dec 12, 2018·Frontiers in Immunology·Nathalie GuriecPi Nyvall Collén
Sep 27, 2020·Veterinary Immunology and Immunopathology·Youngsub LeeHyun S Lillehoj
Apr 4, 2021·Viruses·Joe McKellarCaroline Goujon

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Datasets Mentioned

BETA
AB021154
AB021153
U07868

Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

Alpha Imager 2000 Documentation and Analysis System
Oligo
GENETYX

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