PMID: 237Jul 1, 1975

The internal-alkaline pH gradient, sensitive to uncoupler and ATPase inhibitor, in growing Clostridium pasteurianum

European Journal of Biochemistry
V RiebelingK Jungermann

Abstract

1. The intracellular pH was measured in growing Clostridium pasteurianum with and acid-base equilibrium distribution method. [14C]Dimethyloxazolidinedione, [14]methylamine and [14C]acetic acid were used as "deltapH-indicators". During growth the extracellular pH decreased from 7.1 to 5.1; simultaneously the intracellular pH changed from 7.5 to 5.9. Thus, the intracellular pH was more alkaline than the extracellular pH by 0.4 to 0.8 pH-units. 2. This pH gradient (interior alkaline) was abolished by the proton conductor carbonylcyanide m-chlorophenylhydrazone and the ATPase inhibitor N,N'-dicyclohexylcarbodiimide. The pH gradient could not be demonstrated in cells depleted of an energy substrate. These results suggest that the pH gradient is formed by an ATPase-driven extrusion of protons from the cells rather than by a Donnan potential. 3. Growth of the organism was inhibited by low concentrations of both carbonylcyanide m-chlorophenylhydrazone (5 muM) and dicyclohexylcarbodiimide (5 muM). This finding suggests that the pH gradient is essential for the growing cell as it may be required for substrate accumulation and other types of transport processes.

References

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Citations

Jan 1, 1990·Archives of Microbiology·J B Russell, H J Strobel
Dec 1, 1980·Archives of Microbiology·G OberliesRudolf K Thauer
Dec 25, 2009·Applied Microbiology and Biotechnology·Thaddeus EzejiHans P Blaschek
Jan 1, 1981·Bio Systems·D A Harris
Jan 1, 1985·Biotechnology Advances·L Häggström
Sep 1, 1995·The Journal of Applied Bacteriology·I De SmetW Verstraete
Jul 15, 1977·European Journal of Biochemistry·R Hartmann, D Oesterhelt
Dec 1, 1982·European Journal of Biochemistry·S F BiketovV L Tsuprun
Dec 1, 2010·Applied and Environmental Microbiology·Althea M HoseinCharles E Smith
Jan 4, 2003·Journal of Bacteriology·Karen OlssonGregory M Cook
Jan 1, 1987·Annals of the New York Academy of Sciences·Eleftherios T PapoutsakisM Huesemann
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Jan 15, 1979·Biochemical and Biophysical Research Communications·D Kleiner, E Fitzke
May 1, 1987·Journal of Dairy Science·J B Russell, H J Strobel

Related Concepts

Carbodiimides
Carbonyl Cyanide m-Chlorophenyl Hydrazone
Clostridium
Dicyclohexylcarbodiimide
Energy Metabolism
Hydrogen-Ion Concentration
Nitriles

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