PMID: 8995618Feb 1, 1997Paper

The internal open reading frame within the nucleocapsid gene of mouse hepatitis virus encodes a structural protein that is not essential for viral replication

Journal of Virology
F FischerP S Masters

Abstract

The coronavirus mouse hepatitis virus (MHV) contains a large open reading frame embedded entirely within the 5' half of its nucleocapsid (N) gene. This internal gene (designated I) is in the +1 reading frame with respect to the N gene, and it encodes a mostly hydrophobic 23-kDa polypeptide. We have found that this protein is expressed in MHV-infected cells and that it is a previously unrecognized structural protein of the virion. To analyze the potential biological importance of the I gene, we disrupted its expression by site-directed mutagenesis using targeted RNA recombination. The start codon for I was replaced by a threonine codon, and a stop codon was introduced at a short interval downstream. Both alterations created silent changes in the N reading frame. In vitro translation studies showed that these mutations completely abolished synthesis of I protein, and immunological analysis of infected cell lysates confirmed this conclusion. The MHV I mutant was viable and grew to high titer. However, the I mutant had a reduced plaque size in comparison with its isogenic wild-type counterpart, suggesting that expression of I confers some minor growth advantage to the virus. The engineered mutations were stable during the course of...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Sep 1, 1992·Journal of Virology·S D SenanayakeD A Brian
Oct 25, 1990·Nucleic Acids Research·O Fichot, M Girard
Jan 1, 1990·Annual Review of Microbiology·M M Lai
Jan 1, 1989·Progress in Nucleic Acid Research and Molecular Biology·C E Samuel
Feb 1, 1989·The Journal of Cell Biology·M Kozak
Jan 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·H Ernst, A J Shatkin
Aug 11, 1983·Nucleic Acids Research·M A Skinner, S G Siddell
Aug 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·M W ShawR A Lamb
Jan 1, 1983·Advances in Virus Research·L S Sturman, K V Holmes

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Citations

Sep 29, 2005·Journal of Virology·Cornelis A M de HaanPeter J M Rottier
Dec 13, 2005·Microbiology and Molecular Biology Reviews : MMBR·Susan R Weiss, Sonia Navas-Martin
Jan 22, 2004·Viral Immunology·Sonia Navas-Martin, Susan R Weiss
Dec 17, 2009·Journal of Virology·Timothy J CowleySusan R Weiss
Jul 2, 2020·International Journal of Molecular Sciences·Alejandro LlanesRicardo Lleonart
Jul 24, 2010·Interdisciplinary Perspectives on Infectious Diseases·Jayasri Das Sarma
May 23, 2006·Annual Review of Microbiology·Luis EnjuanesSonia Zuñiga
May 12, 2009·Nature Reviews. Microbiology·Stanley Perlman, Jason Netland
Jul 24, 2012·Molecular Biology and Evolution·Niv SabathDavid Karlin
Jul 19, 2013·Clinical & Developmental Immunology·Dhriti ChatterjeeJayasri Das Sarma
Aug 5, 2020·The Journal of Immunology : Official Journal of the American Association of Immunologists·Lok-Yin Roy WongDong-Yan Jin
Dec 5, 2008·Microbiology and Molecular Biology Reviews : MMBR·Matthew Frieman, Ralph Baric
Feb 28, 2001·Proceedings of the National Academy of Sciences of the United States of America·X Shen, P S Masters
Oct 27, 2017·Proceedings of the National Academy of Sciences of the United States of America·Han DiMargo A Brinton
Sep 11, 1999·Journal of Virology·I J SalanuevaC Risco
Apr 4, 2021·Viruses·Makoto Ujike, Fumihiro Taguchi
Mar 22, 2000·Virology·J B O'Connor, D A Brian
Jun 29, 2018·Journal of Virology·Hazel StewartAndrew E Firth
Jul 17, 2021·Frontiers in Pharmacology·Seyed Abdulmajid AyatollahiNatália Cruz-Martins

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