The intestinal fatty acid binding protein: the role of turns in fast and slow folding processes

Biochemistry
Krishnananda ChattopadhyayCarl Frieden

Abstract

The intestinal fatty acid binding protein is one of a family of proteins that are composed of two beta-sheets surrounding a large interior cavity into which the ligand binds. Glycine residues occur in many of the turns between adjacent antiparallel beta-strands. In previous work, the effect of replacing these glycine residues with valine has been examined with stopped flow instrumentation using intrinsic tryptophan fluorescence spectroscopy [Kim and Frieden (1998) Protein Sci. 7, 1821-1828]. To resolve the burst phase missing in the stopped flow measurements, these valine mutants have been reexamined with sub-millisecond continuous flow instrumentation. Some of the glycine residues have also been replaced with proline, and the folding reactions of these proline mutants have been compared with those of their valine counterparts. In all cases, the stability of the protein is decreased, but some turns appear to be more critical for final structure stabilization than others. Surprisingly, the rate constants observed for all the mutants measured by sub-millisecond continuous flow methods are quite similar (1400-3000 s(-1)), and in all the mutants, there is a shift in the fluorescence emission maximum from that of the unfolded protei...Continue Reading

References

Feb 1, 1996·Journal of Molecular Graphics·R KoradiK Wüthrich

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Citations

Oct 17, 2002·Proceedings of the National Academy of Sciences of the United States of America·Krishnananda ChattopadhyayCarl Frieden
Jul 26, 2002·Proceedings of the National Academy of Sciences of the United States of America·Gregory V Nikiforovich, Carl Frieden
Feb 11, 2005·Proceedings of the National Academy of Sciences of the United States of America·Krishnananda ChattopadhyayCarl Frieden
Nov 5, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Sujit Basak, Krishnananda Chattopadhyay
Feb 24, 2009·Protein Expression and Purification·Kanchan GaraiCarl Frieden
Nov 4, 2008·Archives of Biochemistry and Biophysics·Mariapina D'OnofrioHenriette Molinari
Aug 5, 2005·Proteins·Paula M DalessioIra J Ropson
Nov 25, 2003·Protein Science : a Publication of the Protein Society·Kristofer ModigBertil Halle
Jan 20, 2006·Proteins·Krishnananda Chattopadhyay, Carl Frieden
Feb 16, 2008·Biopolymers·Anna Marie C Marcelino, Lila M Gierasch
Jul 14, 2011·Chemistry, an Asian Journal·Dibyendu Kumar DasKankan Bhattacharyya
May 29, 2004·Journal of Molecular Biology·Kiyoto KamagataKunihiro Kuwajima
Nov 24, 2004·Biophysical Journal·Krishnananda ChattopadhyayCarl Frieden
Dec 18, 2013·Scientific Reports·Sunny SharmaKrishnananda Chattopadhyay
Jun 8, 2006·Journal of the American Chemical Society·Shun HirotaMasahide Terazima

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