PMID: 9636283Oct 1, 1995Paper

The intracellular domain of the rabbit prolactin receptor is able to promote the secretion of a passenger protein via an unusual secretory pathway in lepidopteran cells

Bio/technology
L GarnierM Cerutti

Abstract

We have previously shown that the intracellular domain of the rabbit prolactin receptor (rbPRL-R), lacking typical signal sequences, was very efficiently secreted into the culture medium when expressed in the baculovirus-insect cell system. We have sought to take advantage of this characteristic for secreting cytoplasmic or nuclear proteins. We have constructed a series of recombinant viruses expressing a foreign gene product fused to the intracellular domain of rbPRL-R. Two passenger genes were used, one encoding a cytoplasmic protein (cyclin B) and the other a nuclear protein (cyclin A). The intracellular domain of rbPRL-R was able to promote the export of these two chimeric proteins with a very high efficiency. This new system should prove useful for secretion of proteins which do not require the post-translational modifications of the classical secretory pathway to be fully active.

References

Jan 1, 1991·Annual Review of Cell Biology·D Finley, V Chau
Feb 1, 1990·Biochimie·I B HollandM Blight
Jan 26, 1989·Nature·P L Felgner, G M Ringold
Jan 1, 1986·Annual Review of Cell Biology·P Walter, V R Lingappa
Jan 1, 1993·Recent Progress in Hormone Research·P A KellyJ Finidori

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Citations

Sep 20, 2012·Journal of Cellular Biochemistry·Annunziata Venuto, Ario de Marco
May 6, 2010·Biotechnology and Applied Biochemistry·Richard B HitchmanLinda A King
Sep 29, 2011·Methods : a Companion to Methods in Enzymology·Richard B HitchmanLinda A King

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