The intracellular fate of a recombinant protein is tissue dependent.

Plant Physiology
Georgia DrakakakiEva Stoger

Abstract

Recombinant proteins directed to the secretory pathway in plants require a signal peptide for entry into the endoplasmic reticulum. In the absence of further targeting information, such proteins are generally secreted via the default pathway to the apoplast. This has been well documented in protoplasts and leaf tissue, but the trafficking of recombinant proteins in seeds and other storage tissues has rarely been investigated. We used Aspergillus niger phytase as a model glycoprotein to compare the intracellular fate of a recombinant protein in the leaves and seeds of rice (Oryza sativa). Using fluorescence and electron microscopy we showed that the recombinant protein was efficiently secreted from leaf cells as expected. In contrast, within endosperm cells it was retained in endoplasmic reticulum-derived prolamin bodies and protein storage vacuoles. Consistent with our immunolocalization data, the phytase produced in endosperm cells possessed oligomannose and vacuolar-type N-glycans [Man(3)(Xyl)(Fuc)GlcNAc(2)], whereas the phytase produced in leaves contained predominantly secretion-type N-glycans [GlcNAc(2)Man(3)(Xyl)(Fuc)GlcNAc(2)]. The latter could not be detected in preparations of the endosperm-derived phytase. Our results...Continue Reading

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Citations

Nov 12, 2010·Transgenic Research·Nicolau B CunhaElíbio L Rech
Jul 10, 2013·Journal of Agricultural and Food Chemistry·Anastasia L BodnarManju B Reddy
Jan 9, 2009·Journal of Experimental Botany·Yuhi SaitoTakehiro Masumura
Jun 6, 2009·Journal of Experimental Botany·Lorena MoellerKan Wang
Apr 15, 2010·Plant Physiology·Elsa ArcalisEva Stoger
Feb 9, 2012·Journal of Biomedicine & Biotechnology·Norazlina AhmadIan D Godwin
Jan 18, 2007·Proceedings of the National Academy of Sciences of the United States of America·Bart Van DroogenbroeckGeert De Jaeger
Jan 9, 2009·Bioscience, Biotechnology, and Biochemistry·Takashi MoriShigeru Utsumi
Jan 1, 2008·Biotechnology & Genetic Engineering Reviews·Heribert Warzecha
Feb 2, 2013·Plant Molecular Biology·Kyoko KudoFumio Takaiwa
Dec 4, 2015·Biotechnology Journal·Julia ZischewskiRainer Fischer
Jan 8, 2013·Veterinary Immunology and Immunopathology·María Sol Peréz AguirreburualdeAndrés Wigdorovitz
May 3, 2008·Plant Biotechnology Journal·Meriem BenchabaneDominique Michaud
Dec 6, 2008·Plant Biotechnology Journal·Meriem BenchabaneDominique Michaud
Dec 6, 2008·Plant Biotechnology Journal·Meriem BenchabaneVéronique Gomord
Apr 13, 2011·Plant Biotechnology Journal·Francesca MorandiniMario Pezzotti
Oct 8, 2017·Transgenic Research·Yanaysi CeballoAbel Hernández
May 1, 2009·Functional Plant Biology : FPB·Pablo González-MelendiRita Abranches
Dec 17, 2008·Environmental Biosafety Research·Francesca Pizzuti, Lorenza Daroda
Sep 10, 2008·Journal of Proteome Research·Junling LuoDaichang Yang

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