PMID: 4261038Jul 1, 1972

The intracellular site of synthesis of mitochndrial ribosomal proteins in Neurospora crassa

The Journal of Cell Biology
P M Lizardi, D J Luck

Abstract

The intracellular site of synthesis of mitochondrial ribosomal proteins (MRP) in Neurospora crassa has been investigated using three complementary approaches. (a) Mitochondrial protein synthesis in vitro: Tritium-labeled proteins made by isolated mitochondria were compared to (14)C-labeled marker MRP by cofractionation in a two-step procedure involving isoelectric focusing and polyacrylamide gel electrophoresis. Examination of the electrophoretic profiles showed that essentially none of the peaks of in vitro product corresponded exactly to any of the MRP marker peaks. (b) Sensitivity of in vivo MRP synthesis to chloramphenicol: Cells were labeled with leucine-(3)H in the presence of chloramphenicol, mitochondrial ribosomal subunits were subsequently isolated, and their proteins fractionated by isoelectric focusing followed by gel electrophoresis. The labeling of every single MRP was found to be insensitive to chloramphenicol, a selective inhibitor of mitochondrial protein synthesis. (c) Sensitivity of in vivo MRP synthesis to anisomycin: We have found this antibiotic to be a good selective inhibitor of cytoplasmic protein synthesis in Neurospora. In the presence of anisomycin the labeling of virtually all MRP is inhibited to th...Continue Reading

References

Jul 1, 1968·Archives of Biochemistry and Biophysics·S A Leon, H R Mahler
Sep 1, 1967·Proceedings of the National Academy of Sciences of the United States of America·M R RifkinD J Luck
Mar 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·A W LinnaneH B Lukins
Feb 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·M R Rifkin, D J Luck
Feb 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·G Blobel, D Sabatini
Apr 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·C J Lai, B Weisblum
Feb 3, 1971·Nature: New Biology·P M Lizardi, D J Luck
Jul 7, 1969·Biochemical and Biophysical Research Communications·P J DaveyA W Linnane
Apr 1, 1969·Journal of Molecular Biology·D D Wood, D J Luck
Oct 1, 1964·Proceedings of the National Academy of Sciences of the United States of America·D J Luck, E REICH
Dec 1, 1964·Proceedings of the National Academy of Sciences of the United States of America·P S LEBOYJ G FLAKS

Citations

Jun 24, 1977·Molecular & General Genetics : MGG·H BertrandC A Zagozeski
Jan 1, 1988·Preparative Biochemistry·D M GadboisD M Carlson
Jun 1, 1979·The Journal of Cell Biology·N H Chua, G W Schmidt
May 1, 1976·Biological Reviews of the Cambridge Philosophical Society·J Milner
Aug 1, 1973·The Journal of protozoology·K Rohatgi, S Krawiec
Aug 1, 1973·CRC Critical Reviews in Biochemistry·H R Mahler
Jul 25, 1975·Journal of Molecular Biology·D E Leister, I B Dawid
Mar 1, 1975·Circulation Research·M Rabinowitz, R Zak
Jun 1, 1976·Canadian Journal of Genetics and Cytology. Journal Canadien De Génétique Et De Cytologie·J Kohout, H Bertrand
Feb 27, 1976·Molecular & General Genetics : MGG·A LinI G Wool

Related Concepts

Carbon Isotopes
Cell Fractionation
Cell-Free System
Syntomycin
Actidione
Protoplasm
Electrophoresis, Disc
Isoelectric Focusing
Leucine
Study, Methodological

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