The involvement of cellular recombination and repair genes in RNA-mediated recombination in Saccharomyces cerevisiae.

Genetics
L K Derr

Abstract

We previously demonstrated that a reverse transcript of a cellular reporter gene (his3-AI) can serve as the donor for gene conversion of a chromosomal his3-deltaMscI target sequence, and that this process requires the yeast recombination gene RAD52. In this study, we examine the involvement of other recombination and repair genes in RNA-mediated recombination, and gain insight into the nature of the recombination intermediate. We find that mutation of the mitotic RecA homologs RAD51, RAD55, and RAD57 increases the rate of RNA-mediated recombination relative to the wild type, and that these gene functions are not required for RNA-mediated gene conversion. Interestingly, RAD1 is required for RNA-mediated gene conversion of chromosomal his3-deltaMscI sequences, suggesting that the cDNA intermediate has a region of nonhomology that must be removed during recombination with target sequences. The observation that both RAD1 and RAD52 are required for RNA-mediated gene conversion of chromosomal but not plasmid sequences indicates a clear difference between these two pathways of homologous RNA-mediated recombination.

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Citations

Apr 18, 2012·Biology Direct·Igor B RogozinEugene V Koonin
Feb 18, 2006·Nature Reviews. Genetics·Scott William Roy, Walter Gilbert
Jul 23, 1999·Annals of the New York Academy of Sciences·A Gabriel, E H Mules
Jul 24, 2020·The Journal of Biological Chemistry·Gwendolyn Kaeser, Jerold Chun
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Aug 12, 2005·Cytogenetic and Genome Research·D J Garfinkel
Jan 12, 2005·Proceedings of the National Academy of Sciences of the United States of America·Scott W Roy, Walter Gilbert
Jun 6, 2008·The Journal of Biological Chemistry·Nirotpal Mrinal, Javaregowda Nagaraju
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Jun 8, 2010·Molecular Biology and Evolution·Kazuhiko Ohshima, Kumiko Igarashi
Dec 15, 2007·Genes & Development·Patrick H Maxwell, M Joan Curcio
May 17, 2006·Genome Research·Scott William Roy, Daniel L Hartl

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