The Involvement of Glycosidases in the Cell Wall Metabolism of Suspension-cultured Acer pseudoplatanus Cells.

Plant Physiology
K Keegstra, P Albersheim

Abstract

Several glycosidases have been isolated from suspensioncultured sycamore (Acer pseudoplatanus) cells. These include an alpha-galactosidase, an alpha-mannosidase, a beta-N-acetyl-glucosaminidase, a beta-glucosidase, and two beta-galactosidases. The pH optimum of each of these enzymes was determined. The pH optima, together with inhibition studies, suggest that each observed glycosidase activity represents a separate enzyme. Three of these enzymes, beta-glucosidase, alpha-galactosidase, and one of the beta-galactosidases, have been shown to be associated with the cell surface. The enzyme activities associated with the cell surface were shown to possess the ability to degrade to a limited extent isolated sycamore cell walls. It was found that the activities of beta-glucosidase and of one of the beta-galactosidases increase as the cells go through a period of growth and decrease as cell growth ceases.

References

May 1, 1968·Canadian Journal of Biochemistry·O L Gamborg, D E Eveleigh
Jul 1, 1969·Archives of Biochemistry and Biophysics·A N Heyn
Jun 13, 1967·Biochimica Et Biophysica Acta·M Katz, L Ordin
May 1, 1964·Canadian Journal of Biochemistry·M U AHMED, F S COOK
Jul 1, 1967·Plant Physiology·D J NevinsP Albersheim
Jul 1, 1967·Plant Physiology·S H LeeR S Bandurski
Jun 1, 1968·Plant Physiology·D J NevinsP Albersheim

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Citations

Dec 1, 1974·Planta·V Wiemken-GehrigP Matile
Jul 1, 1975·Plant Physiology·A K Murray, R S Bandurski
Nov 1, 1978·Plant Physiology·B Thomas, J A Webb

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