PMID: 9443937Jan 28, 1998Paper

The involvement of novel protein kinase C isozymes in influencing sensitivity of breast cancer MCF-7 cells to tumor necrosis factor-alpha

Molecular Pharmacology
Alakananda Basu

Abstract

Protein kinase C (PKC) has been implicated in tumor necrosis factor-alpha (TNF) signaling. Structurally and functionally distinct PKC activators and selective inhibitors of PKC were used to investigate the involvement of PKC isozymes in influencing TNF sensitivity in MCF-7 cells. Activators of PKC, such as phorbol-12, 13-dibutyrate (PDBu) (1.0 microM), indolactam V (10 microM), and bryostatin 1 (1.0 microM) decreased the sensitivity of MCF-7 cells to TNF by 5-, 10-, and 1.7-fold, respectively. The PKC-specific inhibitor bisindolylmaleimide II (BIM) (> or = 1 microM) antagonized the effect of PDBu in protecting MCF-7 cells against TNF cytotoxicity. High concentrations of BIM (> or = 10 microM) also significantly enhanced the sensitivity of MCF-7 cells to TNF. In contrast, Gö 6976, a specific inhibitor of cPKCs, did not potentiate TNF sensitivity and failed to reverse the effect of PDBu. In addition, BIM but not Gö 6976 blocked PDBu-mediated down-regulation of TNF receptors. There was no correlation between down-regulation of PKC alpha, -delta, and -epsilon, and protection against TNF cytotoxicity by PKC activators. A 6-hr exposure to 1.0 microM PDBu, 10 microM indolactam V, and 1.0 microM bryostatin 1 caused a 1.8-, 3.5- and 1.2...Continue Reading

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Citations

Oct 9, 1998·The Journal of Biological Chemistry·M S ResnickC S Hahn
Jul 22, 2005·The Journal of General Virology·Natalia KucićPero Lucin
Feb 20, 2019·Cancers·Alakananda Basu
Feb 26, 2013·Histochemistry and Cell Biology·Michitaka SuzukiToyoshi Fujimoto
Nov 18, 2014·Biochemical Society Transactions·Udi ZurgilEtta Livneh
May 21, 2014·International Journal of Oncology·Deepanwita Pal, Alakananda Basu
Sep 2, 2008·Naunyn-Schmiedeberg's Archives of Pharmacology·Chih Chung Chou, Chen Ya Hsu
Feb 13, 2001·Biochemical and Biophysical Research Communications·A BasuB Sun
Dec 9, 2000·Biochemical and Biophysical Research Communications·G R Akkaraju, A Basu
Apr 14, 2016·Organic Letters·Jeremy Haynes-SmithKelvin L Billingsley
Apr 26, 2011·Experimental Cell Research·Ana TamarkinEtta Livneh

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