The involvement of phosphatidylinositol 3-kinase in gonadotropin-releasing hormone-induced gonadotropin alpha- and FSHbeta-subunit genes expression in clonal gonadotroph LbetaT2 cells

Molecular and Cellular Endocrinology
Sandra MutiaraKohji Miyazaki

Abstract

Akt/protein kinase B (Akt/PKB), which is activated by phosphatidylinositol-3 kinase (PI3-kinase), plays an important role in cell survival and cell proliferation. Using the well differentiated, clonal gonadotroph cell line, LbetaT2, we examined (1) whether Akt/PKB was activated by gonadotropin-releasing hormone (GnRH); (2) the contribution of PI3-kinase-Akt/PKB pathway in each of gonadotropin subunit gene expression; (3) crosstalk between extracellular signal-regulated kinase (ERK) and Akt/PKB pathways. Insulin-like growth factor-1 (IGF-1) was used as Akt/PKBs classic activator. Western blot analyses using antibodies specific for the phosphorylated forms of ERK and Akt/PKB demonstrated that both were rapidly phosphorylated following treatment with GnRH and IGF-1. Akt/PKB activation by GnRH and IGF-1 was completely eliminated in the presence of the PI3-kinase inhibitor, LY 294002, but not in the presence of an Akt/PKB inhibitor. Interestingly, the total amount of Akt/PKB protein was dramatically increased in the presence of LY 294002. Phosphorylation of ERK was significantly increased in the presence of LY 294002 alone, and was further increased when GnRH was used in combination with LY 294002. In experiments using a luciferase ...Continue Reading

References

Oct 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·J Q ChengJ R Testa
May 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·P F JonesB A Hemmings
Jan 1, 1989·Archives of Gynecology and Obstetrics·T MatsudaT Tamaya
Apr 1, 1995·Endocrine Reviews·D LeRoithC T Roberts
Apr 1, 1994·Acta Paediatrica. Supplement·P J Trainer
Dec 19, 1995·Proceedings of the National Academy of Sciences of the United States of America·U B KaiserW W Chin
Apr 30, 1998·Current Opinion in Cell Biology·J Downward
Mar 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·I PályiR F Murphy
Jan 27, 2000·Cytokine & Growth Factor Reviews·B R LackeyD M Henricks
Mar 10, 2001·The Journal of Clinical Endocrinology and Metabolism·Y KidoD Accili
Jun 5, 2003·Molecular and Cellular Endocrinology·Hadas LewyZ Naor
May 28, 2005·Journal of Cellular Physiology·Bukhtiar H ShahKevin J Catt
Jun 24, 2005·AJR. American Journal of Roentgenology·Aditya DaftarySanjay Saluja
Feb 17, 2006·Biology of Reproduction·Heather A Ferris, Margaret A Shupnik
Jun 3, 2006·Journal of Cellular Physiology·Adrienne NeithardtBukhtiar H Shah

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Citations

Nov 1, 2008·Molecular Endocrinology·Minh-Ha T DoMark A Lawson
Dec 6, 2012·Molecular Endocrinology·Qian WangStuart C Sealfon

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