The involvement of RhoA and Wnt-5a in the tumorigenesis and progression of ovarian epithelial carcinoma

International Journal of Molecular Sciences
Shuo ChenYang Zhao

Abstract

Ras homolog gene family member A (RhoA) is involved in Wnt-5a-induced migration of gastric and breast cancer cells. We investigated the roles of RhoA and Wnt-5a in ovarian carcinoma. RhoA and Wnt-5a mRNA and protein expression in normal fallopian tube epithelium, benign tumors, primary ovarian carcinomas, and metastatic omentum were quantified. RhoA or Wnt-5a was knocked down in OVCAR3 ovarian carcinoma cells using siRNAs and cell phenotype and expression of relevant molecules were assayed. RhoA and Wnt-5a mRNA and protein expression were found to be significantly higher in metastatic omentum than in ovarian carcinomas, benign tumors, and normal fallopian tube epithelium (p < 0.05), and positively associated with differentiation and FIGO staging (stage I/II vs. stage III/IV) in ovarian carcinoma (p < 0.05). RhoA and Wnt-5a expression were positively correlated in ovarian carcinoma (p = 0.001, R2 = 0.1669). RhoA or Wnt-5a knockdown downregulated RhoA and Wnt-5a expression; reduced cell proliferation; promoted G1 arrest and apoptosis; suppressed lamellipodia formation, cell migration, and invasion; and reduced PI3K, Akt, p70S6k, Bcl-xL, survivin, and VEGF mRNA or protein expression. This is the first demonstration that RhoA and W...Continue Reading

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Citations

Jun 1, 2014·International Journal of Molecular Sciences·Gangping WangYanqing Sun
Apr 22, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Changmei Liu, Fangmei Yang
Sep 28, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Jing WangXin Wu
Jul 30, 2014·Seminars in Cancer Biology·Peter L SternOwen J McGinn
Apr 17, 2018·International Journal of Molecular Sciences·Akio ShimizuHisakazu Ogita
Jul 12, 2017·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Ben-Gang WangQian Xu
Aug 11, 2018·Endocrine-related Cancer·Amy CoopesCaroline Elizabeth Ford
May 14, 2020·Journal of Cellular Physiology·Baoping XuYang Yang
Aug 23, 2017·Nature Communications·Monika HaemmerleAnil K Sood
Sep 17, 2016·International Journal of Oncology·Shujie ChengDaniel Sliva
Jan 29, 2019·The Tohoku Journal of Experimental Medicine·Mahy EgizNobuo Yaegashi
May 8, 2020·Analytical Cellular Pathology (Amsterdam)·Nada El BabaMirvat El-Sibai
Mar 27, 2019·International Journal of Molecular Sciences·Laura MoffittMaree Bilandzic

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Methods Mentioned

BETA
transfection
PCR
flow cytometry
surgical resection
protein assay
X-ray

Software Mentioned

ImageQuant
SPSS
Image J

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