The involvement of serum exosomal miR-500-3p and miR-770-3p in aging: modulation by calorie restriction

Oncotarget
Eun Kyeong LeeHae Young Chung

Abstract

Recent studies have shown a role for miRNAs in aging and age-related diseases, and the modulation of miRNA expression by diet attracts attention as a new therapeutic strategy. Here, we focused on identifying specific exosomal miRNAs derived from serum of aged rats and the effect of short-term calorie restriction (CR) on their expression. Exosomes from serum of young (7-month), old (22-month), and old-CR Sprague Dawley rats were isolated and characterized by transmission electron microscopy analyses, dynamic light scattering measurements, and Western blotting. A total of 12 significantly expressed miRNAs in serum exosomes of young and old rats were identified by next generation sequencing. After analysis of qRT-PCR, we found that miR-500-3p and miR-770-3p expression was significantly upregulated by aging and downregulated by CR. Furthermore, receiver operating characteristic (ROC) curve revealed that the selected miRNAs represented high accuracy in discriminating old rats from young rats. Finally, PANTHER analysis predicted selected miRNAs targets genes involved in Wnt/chemokines and cytokines -related inflammatory signaling pathway and function as transcription factor. In conclusion, our results suggest that the expression of s...Continue Reading

References

Jun 1, 2002·Science·Randall T MoonNorbert Perrimon
May 15, 2004·Nature Reviews. Genetics·David P Bartel, Chang-Zheng Chen
Jan 18, 2008·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Nathan A DeCarolisAmelia J Eisch
Jul 30, 2008·Proceedings of the National Academy of Sciences of the United States of America·Patrick S MitchellMuneesh Tewari
Sep 28, 2010·BMC Genomics·Márcia M AranhaCecília M P Rodrigues
Nov 26, 2010·Circulation Research·Atsuhiko T NaitoIssei Komuro
Mar 6, 2012·Nature Methods·Ben Langmead, Steven L Salzberg
Dec 4, 2012·Experimental Gerontology·K W ChungH Y Chung
Apr 16, 2013·Nature Reviews. Drug Discovery·Samir EL AndaloussiMatthew J A Wood
Sep 7, 2013·Journal of Extracellular Vesicles·Nina GennebäckStellan Mörner
Mar 13, 2014·Ageing Research Reviews·Joseph M Dhahbi
Apr 29, 2014·Current Opinion in Immunology·Fabiola OlivieriRuth R Montgomery
May 17, 2014·Ageing Research Reviews·Joshua D PalmerNicole L Simone
Aug 29, 2014·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Haiyang ZhangDonghai Li
Oct 30, 2014·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Ileana BadiAngela Raucci
Sep 5, 2015·Renal Failure·A Gutiérrez-EscolanoF Gómez-Pérez
Sep 16, 2015·International Journal of Molecular Sciences·Tatsuya MachidaManabu Morita
Nov 3, 2015·Osteoarthritis and Cartilage·M A Greene, R F Loeser
May 12, 2016·Scientific Reports·Eduardo Andrés-LeónAna M Rojas

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Methods Mentioned

BETA
transmission electron microscopy
dynamic light scattering
chip
RNA Seq
PCR

Software Mentioned

Seq
miARma
FeatureCounts
Cutadapter
PANTHER
GraphPad Prism
TargetScan
Bowtie2

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