Abstract
Epoxide hydrolase hydrates epoxides to vicinal diols in the phyto-oxylipin peroxygenase pathway resulting in the production of epoxy alcohols, dihydrodiols, triols and epoxides, including many lipid epoxides associated with resistance. Two epoxide hydrolase genes from Nicotiana benthamiana L., NbEH1.1 and NbEH1.2, were amplified from coding DNA of leaves during a susceptible response to the hemibiotrophic pathogens, Colletotrichum destructivum O'Gara, Colletotrichum orbiculare Berk. and Mont. von Arx. or Pseudomonas syringae pv. tabaci Wolf and Foster, or the hypersensitive resistance response to P. syringae pv. tabaci expressing avrPto. Increases in expression of NbEH1.1 generally occurred during the late biotrophic and necrotrophic stages in the susceptible responses and before the hypersensitive response. NbEH1.2 expression was not significantly induced by C. orbiculare but was induced by C. destructivum, P. syringae pv. tabaci and P. syringae pv. tabaci expressing avrPto, although to a lesser degree than NbEH1.1. Virus-induced gene silencing of NbEH1.1 delayed the appearance of lesions for C. destructivum, reduced populations of P. syringae pv. tabaci and increased populations of P. syringae pv. tabaci expressing avrPto. Th...Continue Reading
References
Mar 1, 1992·Journal of Bacteriology·P C RonaldB J Staskawicz
Oct 1, 1995·The Plant Cell·C M RommensB J Staskawicz
Jan 1, 1995·DNA and Cell Biology·J K BeethamB D Hammock
Aug 1, 1994·The Plant Journal : for Cell and Molecular Biology·A StapletonW R Belknap
Jan 1, 1993·Cancer·G I MurrayM D Burke
Oct 9, 1998·Progress in Lipid Research·E Blée
Oct 21, 1998·The Plant Journal : for Cell and Molecular Biology·A GuoD F Klessig
May 14, 1999·Current Opinion in Plant Biology·D C Baulcombe
Apr 28, 2000·European Journal of Biochemistry·M ArahiraC Fukazawa
Nov 10, 2001·Annual Review of Phytopathology·Ray Hammerschmidt
May 25, 2002·The Plant Journal : for Cell and Molecular Biology·Yule LiuS P Dinesh-Kumar
Jul 18, 2002·Trends in Plant Science·Elizabeth Blée
Jul 18, 2002·Proceedings of the National Academy of Sciences of the United States of America·Eva VranováWim Van Camp
Mar 1, 1996·Plant Physiology·M. Hamberg, G. Hamberg
Mar 1, 1992·The Plant Cell·J. MalamyD. F. Klessig
Mar 1, 2003·Journal of Microbiological Methods·N ChenP H Goodwin
Jun 26, 2003·Nucleic Acids Research·Ramu ChennaJulie D Thompson
Oct 11, 2003·The Journal of Biological Chemistry·Cornelia GobelSabine Rosahl
Feb 6, 2004·Plant Molecular Biology·Kenji GomiKazuya Akimitsu
Jul 9, 2004·The EMBO Journal·Fangming XiaoJian-Min Zhou
Sep 17, 2004·Nature·David Baulcombe
Sep 21, 2004·Annual Review of Plant Biology·Dominique Robertson
Oct 21, 2004·The Plant Cell·Kohki YoshimotoYoshinori Ohsumi
Oct 29, 2004·Plant & Cell Physiology·Ken MatsuokaHiroo Fukuda
Mar 8, 2005·Progress in Lipid Research·John W NewmanBruce D Hammock
May 3, 2005·Annual Review of Plant Biology·Mary Beth Mudgett
Sep 29, 2005·Journal of Experimental Botany·Nathalie NicotDanièle Evers
Nov 22, 2005·Plant Physiology·Isabelle ProstJoëlle Fournier
Feb 4, 2006·Plant Cell Reports·Agnes SzatmariZoltán Bozsó
Sep 1, 1991·Plant Physiology·H OhtaY Morita
Jul 13, 2007·Current Opinion in Plant Biology·Luis da CunhaDavid Mackey