PMID: 8584437Jan 1, 1996Paper

The ion conductances of colonic crypts from dexamethasone-treated rats

Pflügers Archiv : European journal of physiology
D EckeR Greger

Abstract

Whole-cell patch-clamp studies were performed in isolated colonic crypts of rats pretreated with dexamethasone (6 mg/kg subcutaneously on 3 days consecutively prior to the experiment). The cells were divided into three categories according to their position along the crypt axis: surface cells (s.c.); mid-crypt cells (m.c.) and crypt base cells (b.c.). The zero-current membrane voltage (Vm) was -56 +/- 2 mV in s.c (n = 34); -76 +/- 2 mV in m.c. (n = 47); and -87 +/- 1 mV in b.c. (n = 87). The whole-cell conductance (Gm) was similar (8-12 nS) in all three types of cells. A fractional K+ conductance accounting for 29-67% of Gm was present in all cell types. A Na+ conductance was demonstrable in s.c. by the hyperpolarizing effect on Vm of a low-Na+ (5 mmol/l) solution. In m.c. and b.c. the hyperpolarizing effect was much smaller, albeit significant. Amiloride had a concentration-dependent hyperpolarizing effect on Vm in m.c. and even more so in s.c.. It reduced Gm by approximately 12%. The dissociation constant (KD) was around 0.2 micromol/l. Triamterene had a comparable but not additive effect (KD = 30 micromol/l, n = 14). Forskolin (10 micromol/l, in order to enhance cytosolic adenosine 3', 5'-cyclic monophosphate or cAMP) depola...Continue Reading

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Citations

Jan 1, 1996·Pflügers Archiv : European journal of physiology·D EckeR Greger
Apr 23, 2010·The Journal of Membrane Biology·Akihiro InagakiToru Ishikawa
Mar 17, 2010·Pflügers Archiv : European journal of physiology·Nina HimmerkusMarkus Bleich
Dec 5, 2002·European Journal of Pharmacology·Gisela KuhnErwin Scharrer
Oct 22, 2004·European Journal of Pharmacology·Rainer CermakGisela Kuhn
Jan 11, 2019·The Journal of Immunology : Official Journal of the American Association of Immunologists·Carolina SerranoPorfirio Nava
Jul 9, 2005·American Journal of Physiology. Gastrointestinal and Liver Physiology·Robert R CimaDavid I Soybel
Apr 1, 1996·Pflügers Archiv : European journal of physiology·D EckeR Greger
May 1, 1996·Pflügers Archiv : European journal of physiology·R WarthR Greger
Jan 5, 2002·Physiological Reviews·Karl Kunzelmann, Marcus Mall
Oct 1, 1996·Pflügers Archiv : European journal of physiology·M BleichR Greger
Oct 29, 2000·American Journal of Physiology. Gastrointestinal and Liver Physiology·G M FraserB Schwartz
Oct 25, 2003·American Journal of Physiology. Cell Physiology·A InagakiT Ishikawa
Dec 9, 1998·The American Journal of Physiology·M MallK Kunzelmann
May 18, 1999·Biochemical and Biophysical Research Communications·A IkariN Takeguchi

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