PMID: 697734Aug 1, 1978Paper

The iron-binding properties of hen ovotransferrin

The Biochemical Journal
J WilliamsK Moreton

Abstract

1. The distribution of iron between the two iron-binding sites in partially saturated ovotransferrin was studied by labelling with 55Fe and 59Fe and by gel electrophoresis in a urea-containing buffer. 2. When iron is added in the form of chelate complexes at alkaline pH, binding occurs preferentially at the N-terminal binding site. In acid, binding occurs preferentially at the C-terminal site. 3. When simple iron donors (ferric and ferrous salts) are used the metal is distributed at random between the binding sites, as judged by the gel-electrophoresis method. The double-isotope method shows a preference of ferrous salts for the N-terminal site. 4. Quantitative treatment of the results of double-isotope labelling suggests that in the binding of iron to ovotransferrin at alkaline pH co-operative interactions between the sites occur. These interactions are apparently absent in the displacement of copper and in the binding of iron at acid pH.

Citations

Sep 3, 2010·International Journal for Parasitology·Bradley T HeronChristopher D Huston
Aug 3, 2001·Biologicals : Journal of the International Association of Biological Standardization·L von BonsdorffJ Parkkinen
Nov 4, 2004·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·David J LeeheyRajiv Agarwal
Feb 29, 1984·Biochemical and Biophysical Research Communications·T YamamuraK Satake
Feb 1, 1982·European Journal of Biochemistry·J M Jeltsch, P Chambon
Sep 1, 1986·The Proceedings of the Nutrition Society·J H Brock, T Mainou-Fowler
Jan 1, 1985·The Journal of Membrane Biology·W S May, P Cuatrecasas
Feb 15, 1980·Klinische Wochenschrift·H Vorherr
Nov 10, 2000·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·J ParkkinenK Rosenlöf
Jan 1, 1981·Animal Blood Groups and Biochemical Genetics·A M Rashid
Sep 25, 1999·The Journal of Biological Chemistry·H KurokawaM Hirose
Mar 27, 2001·FEMS Microbiology Letters·S MatinahoJ Parkkinen
Apr 15, 2017·Evidence-based Complementary and Alternative Medicine : ECAM·Constantin ChiurciuRomeo Teodor Cristina
Feb 28, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Kumiko HaradHiroaki Okabe
Apr 1, 1987·Journal of Inorganic Biochemistry·M S CheukW M Keung

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