The irradiation of rabbit sperm cells with He-Ne laser prevents their in vitro liquid storage dependent damage

Animal Reproduction Science
Nicolaia IaffaldanoS Passarella

Abstract

The aim of the study was to investigate the effects of different energy doses of helium-neon (He-Ne) laser irradiation on both mitochondrial bioenergetics functions and functional quality of rabbit spermatozoa during 48 h of in vitro liquid storage at 15 degrees C. 11 rabbit semen pools were each divided into four aliquots: three of them were irradiated with He-Ne laser with different energy doses (3.96, 6.12 and 9.00 J/cm(2)) being the last control kept under the same experimental conditions without irradiation. Sperm motility, viability and acrosome integrity were monitored together with cytochrome c oxidase (COX) activity and the cell energy charge (EC) at 0, 24 and 48 h of storage. Irradiated samples stored for 24 and 48 h better maintained motility (P < 0.01), acrosome integrity (P < 0.01) and viability (P < 0.05) with respect to the control, particularly with the energy dose of 6.12 J/cm(2) that showed the most intense biostimulative effect. COX activity and EC were immediately increased by irradiation particularly in the treatments 6.12 and 9.00 J/cm(2) (P < 0.05), that maintained their levels higher with respect to the control after 48 h of storage (P < 0.01). COX activity of rabbit sperm cells was positively correlated...Continue Reading

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Citations

May 4, 2012·Photomedicine and Laser Surgery·Tiina I Karu
Feb 15, 2013·Lasers in Medical Science·Reza Salman YazdiMohammad Ali Sadighi Gilani
Jun 9, 2016·Lasers in Medical Science·Adriano F P SiqueiraMayra E O A Assumpção
Apr 21, 2017·Scientific Reports·Daryl PreeceMichael W Berns
Sep 3, 2017·Polish Journal of Veterinary Sciences·G PalubinskasR Antanaitis
Nov 5, 2019·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·M Ríos-RamírezH Mercado-Uribe
Jun 3, 2020·Lasers in Medical Science·Luisa ZupinGiuseppe Ricci
May 29, 2018·BioMedicine·Sergey Vladimirovich Moskvin, Oleg Ivanovich Apolikhin
Feb 2, 2021·Journal of Photochemistry and Photobiology. B, Biology·V Yu PlavskiiV A Orlovich
Jan 13, 2021·Animals : an Open Access Journal From MDPI·Jaime CatalánJordi Miró
Apr 2, 2021·Lasers in Surgery and Medicine·Burkhard T EspeyKatrin van der Ven
Jan 12, 2011·Theriogenology·Z Abdel-SalamM A Harith

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