PMID: 8953384Dec 1, 1996Paper

The irreversibility of inner mitochondrial membrane permeabilization by Ca2+ plus prooxidants is determined by the extent of membrane protein thiol cross-linking

Journal of Bioenergetics and Biomembranes
Roger F CastilhoA E Vercesi

Abstract

We have previously shown that mitochondrial membrane potential (delta psi) drop promoted by prooxidants and Ca2+ can be reversed but not sustained by ethylene glycol-bis(beta-aminoethylether)-N,N,N',N'-tetraacetic acid (EGTA) unless dithiothreitol (DTT), a disulfide reductant, is also added [Valle, V. G. R., Fagian, M. M., Parentoni, L. S., Meinicke, A. R., and Vercesi, A. E. (1993). Arch. Biochem. Biophys. 307, 1-7]. In this study we show that catalase or ADP are also able to potentiate this EGTA effect. When EGTA is added long after (12 min) the completion of swelling or delta psi elimination, no membrane resealing occurs unless the EGTA addition was preceded by the inclusion of DTT, ADP, or catalase soon after delta psi was collapsed. Total delta psi recovery by EGTA is obtained only in the presence of ADP. The sensitivity of the ADP effect to carboxyatractyloside strongly supports the involvement of the ADP/ATP carrier in this mechanism. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of solubilized membrane proteins shows that protein aggregation due to thiol cross-linkage formed during delta psi drop continues even after delta psi is already eliminated. Titration with 5,5'-dithio-bis(2-nitrobenzoic acid) support...Continue Reading

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Citations

Feb 3, 2011·Journal of Bioenergetics and Biomembranes·R A P CostaN C Souza-Pinto
Oct 4, 2011·Journal of Bioenergetics and Biomembranes·Juliana A RonchiRoger F Castilho
Jun 28, 2005·Archives of Biochemistry and Biophysics·Gilberto Lázaro Pardo AndreuAnibal E Vercesi
Jan 23, 1999·Free Radical Biology & Medicine·A J Kowaltowski, A E Vercesi
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Dec 19, 2012·Antioxidants & Redox Signaling·Tiago R FigueiraAnibal E Vercesi
Apr 24, 2007·Free Radical Biology & Medicine·Irina G Kessova, Arthur I Cederbaum
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Apr 5, 2020·International Journal of Molecular Sciences·Paola Venditti, Sergio Di Meo
Apr 19, 2005·Chemical Research in Toxicology·Fernando DutraEtelvino J H Bechara
Sep 17, 2004·Archives of Biochemistry and Biophysics·Fernando Dutra, Etelvino J H Bechara
Jul 9, 2021·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Sergey M Korotkov
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