PMID: 8947550Dec 1, 1996Paper

The isoforms of proprotein convertase PC5 are sorted to different subcellular compartments

The Journal of Cell Biology
I De BieN G Seidah

Abstract

The proprotein convertase PC5 is encoded by multiple mRNAs, two of which give rise to the COOH-terminal variant isoforms PC5-A (915 amino acids [aa]) and PC5-B (1877 aa). To investigate the differences in biosynthesis and sorting between these two proteins, we generated stably transfected AtT-20 cell lines expressing each enzyme individually and examined their respective processing pattern and subcellular localization. Biosynthetic analyses coupled to immunofluorescence studies demonstrated that the shorter and soluble PC5-A is sorted to regulated secretory granules. In contrast, the COOH-terminally extended and membrane-bound PC5-B is located in the Golgi. The presence of a sorting signal in the COOH-terminal 38 amino acids unique to PC5-A was demonstrated by the inefficient entry into the regulated secretory pathway of a mutant lacking this segment. EM of pancreatic cells established the presence of immunoreactive PC5 in glucagon-containing granules, demonstrating the sorting of this protein to dense core secretory granules in endocrine cells. Thus, a single PC5 gene generates COOH-terminally modified isoforms with different sorting signals directing these proteins to distinct subcellular localization, thereby allowing them t...Continue Reading

References

Sep 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·A RehemtullaR J Kaufman
Nov 1, 1992·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·C ScalaG C Manara
Dec 1, 1992·Current Opinion in Cell Biology·G W Hart
Aug 11, 1992·Proceedings of the National Academy of Sciences of the United States of America·S J ChanD F Steiner
Jul 1, 1992·Molecular Endocrinology·O Vindrola, I Lindberg
Dec 1, 1991·The Journal of Cell Biology·S A ToozeW B Huttner
Mar 1, 1991·The Journal of Cell Biology·L Matsuuchi, R B Kelly
Feb 1, 1988·The Journal of Cell Biology·L MatsuuchiR B Kelly
Dec 1, 1987·The Journal of Cell Biology·P A Baeuerle, W B Huttner
Jan 1, 1987·Annual Review of Cell Biology·T L Burgess, R B Kelly
Jan 1, 1982·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M Bendayan
May 1, 1995·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·E R LeeJ S Mort
Jan 1, 1995·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·D MalideM Bendayan
Apr 29, 1994·Biochemical and Biophysical Research Communications·A TsujiY Matsuda
Aug 1, 1993·Current Opinion in Cell Biology·R Bauerfeind, W B Huttner
Jan 1, 1993·Annual Review of Cell Biology·I S TrowbridgeC R Hopkins
Jul 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·J LussonN G Seidah
Apr 16, 1996·Proceedings of the National Academy of Sciences of the United States of America·N G SeidahR Day
Jun 1, 1996·The Journal of Cell Biology·R A ChavezH P Moore

❮ Previous
Next ❯

Citations

Jun 8, 2000·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·C Thacker, A M Rose
Mar 14, 2002·Molecular Reproduction and Development·Becky S Y WongDerrick E Rancourt
May 12, 2006·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Patrick Kitabgi
Mar 14, 2007·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Nabil G Seidah, Annik Prat
Mar 10, 2005·Virchows Archiv : an International Journal of Pathology·Philipp StawowyKristof Graf
Jun 6, 2003·Progress in Neurobiology·Volkmar LessmannMarzia Malcangio
Nov 14, 1997·Current Opinion in Biotechnology·N G Seidah, M Chrétien
Dec 18, 2001·The Biochemical Journal·Nino MzhaviaLloyd D Fricker
Dec 30, 2011·The New England Journal of Medicine·Andrew W Artenstein, Steven M Opal
Apr 21, 2009·Proceedings of the National Academy of Sciences of the United States of America·Jimmy D DikeakosJames G Omichinski
Jan 21, 2011·The Journal of Biological Chemistry·Ralf M LeonhardtPeter Cresswell
Jun 28, 2011·The Journal of Biological Chemistry·Xiaowei SunNabil G Seidah
Feb 2, 2012·The Journal of Biological Chemistry·Jeroen DeclercqJohn W M Creemers
Mar 9, 2000·Journal of Virology·F DenisR P Sékaly
Dec 16, 2005·Molecular and Cellular Biology·Rachid EssalmaniAnnik Prat
May 10, 2008·Endocrinology·Sang-Nam Lee, Iris Lindberg
Aug 18, 2006·Biological Chemistry·Nabil G SeidahAnnik Prat
May 2, 2003·Proceedings of the National Academy of Sciences of the United States of America·Kazuya UedaDonald F Steiner
Feb 17, 1999·Proceedings of the National Academy of Sciences of the United States of America·N G SeidahM Marcinkiewicz
Jul 30, 2002·Proceedings of the National Academy of Sciences of the United States of America·Xiaorong ZhuDonald F Steiner
Jul 24, 2002·Proceedings of the National Academy of Sciences of the United States of America·Xiaorong ZhuDonald F Steiner

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.