Jul 1, 1993

The isolated and perfused brain of the guinea-pig in vitro

The European Journal of Neuroscience
M MühlethalerR Llinás

Abstract

We describe here an isolated and perfused in vitro adult guinea-pig whole brain preparation which is an extension of the previously described in vitro brainstem-cerebellum preparation. Viability was tested by the analysis of trans-synaptic responses along the visual pathways following the electrical stimulation of the optic nerve or the optic radiations. The evoked field potentials were recorded in the dorsal lateral geniculate, the superior colliculus and the visual cortex. The distribution of extracellular currents was studied using current source density analysis, in order to determine the amplitude, time course and spatial organization of the synaptic activity at these sites. The study indicates that field potentials were very similar to those described in vivo. These data demonstrate the survival of a complex adult sensory system in vitro and suggest that this preparation can be used for the analysis of multisynaptic circuits in the mammalian brain.

  • References28
  • Citations96

References

  • References28
  • Citations96

Citations

Mentioned in this Paper

Optic Tract Structure
Optic Lobe, Human
Entire Optic Nerve
Entire Brainstem
Extracellular
Cavia
Brain
Perfusion
Subcortical Sensory Systems
Potentials, Event-Related

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