PMID: 6109549Nov 20, 1980Paper

The isolation and characterisation of guinea-pig polymorphonuclear leucocyte actin and myosin

Biochimica Et Biophysica Acta
N CrawfordP Jackson

Abstract

The contractile proteins actin and myosin have been isolated from the soluble phase of guinea-pig polymorphonuclear leucocytes and partially characterised. Two forms of actin have been identified, designated 'Mg-actin' and 'KCl-actin'. They have different polymerising properties but their propensity to form synthetic homologous and heterologous actomyosins and to inhibit DNAase-1 does not significantly differ. Both show beta and gamma isoelectric forms in focusing gels and the Mg-actin accounts for about 5% of the soluble-phase protein and te KCl-actin around 2%. Leucocyte myosin has been isolated by affinity chromatography on N6-ADP-Sepharose with a good enrichment of both Ca2+-ATPase and the ATPase activity measured in the absence of Ca2+ or Mg2+ and in the presence of EDTA. This protein, too, has the capacity to form synthetic homologous and hybrid actomyosins with enhancement of the basal Mg2+-ATPase activity. The ratio of actin to myosin in the leucocyte calculated on a molar basis is well in excess of 100, a figure consistent with the findings from other non-muscle cells.

References

Jan 1, 1976·Biochemical Society Transactions·P Jackson, N Crawford
Sep 27, 1977·Biochimica Et Biophysica Acta·A G Castle, N Crawford
Mar 1, 1975·Archives of Biochemistry and Biophysics·J W AbramowitzT C Detwiler
Dec 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·E Lazarides, U Lindberg
Feb 16, 1973·Biochimica Et Biophysica Acta·G L Harris, N Crawford
Sep 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·M ElzingaR S Adelstein
Jul 1, 1972·The Biochemical Journal·P Cummins, S V Perry
Jan 1, 1973·Analytical Biochemistry·M J BlackburnR W Watts
Sep 1, 1973·Analytical Biochemistry·D Bray, S M Brownlee
Jun 27, 1967·Biochimica Et Biophysica Acta·G Bailin, M Bárány
Feb 1, 1938·The Biochemical Journal·I Berenblum, E Chain

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Citations

Nov 16, 2001·Animal Health Research Reviews·J D DubreuilM Gottschalk

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