The isolation and characterisation of antiplatelet antibodies

European Journal of Haematology
Mohsen HamidpourNigel Lindsey

Abstract

The isolation and characterisation of antiplatelet antibodies in autoimmune thrombocytopenia purpura patients (ITP) is described. Autoimmune thrombocytopenia purpura is an autoimmune disease, clinically defined by low platelet counts, normal or increased megakaryocytopoiesis and antiplatelet antibodies in serum. This study used phage display to isolate Fab antiplatelet antibodies to study the structure-function relationships of pathogenic antibodies in ITP. Out of six randomly selected colonies, four colonies reacted strongly with whole platelets in enzyme-linked immunosorbent assay (ELISA). Sequence analysis showed that all four colonies had the same DNA sequence and were the same antibody. Results of Western blotting against non-reduced human platelet lysate showed that the Fab reacted with platelet proteins with apparent molecular weights of 116, 92 and 39 kD. Furthermore, Western blotting assay against purified membrane glycoprotein IIIa demonstrated reactivity against a band with a molecular weight of 92 kD. Results from Western blotting against platelet lysate and pure platelet glycoprotein confirmed the Fab fragment recognised the platelet glycoprotein IIIa. Three out of the four phage colonies produced soluble Fab, whic...Continue Reading

References

Jan 1, 1992·Seminars in Thrombosis and Hemostasis·D J Christie, C D Swinehart
Sep 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·C F BarbasS J Benkovic
May 1, 1989·Electrophoresis·J A Ceglarek, A Revzin
Mar 15, 1995·Experientia·E Beutler
May 1, 1995·Immunology Today·G P Cook, I M Tomlinson
Jan 1, 1995·Blood Cells, Molecules & Diseases·E BeutlerP Lee
Feb 17, 2000·Seminars in Hematology·N S Young
Jan 10, 2003·Clinical and Experimental Immunology·M BehrendtN J Lindsey
Oct 1, 1952·Journal of the Indian Medical Association

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Citations

Sep 26, 2008·Journal of Virology·Gerlinde R Van de WalleNikolaus Osterrieder
Dec 14, 2006·American Journal of Medical Genetics. Part a·M L KulkarniPreethi M Kulkarni
Jun 6, 2018·Clinical Hemorheology and Microcirculation·Mohsen HamidpourRafie Hamidpour

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