Jul 1, 1986

The isolation and characterization of ngm2, a mutation that affects nitrosoguanidine mutagenesis in yeast

Molecular & General Genetics : MGG
P E Nisson, C W Lawrence

Abstract

We have isolated and characterized a new mutant of Saccharomyces cerevisiae, carrying a single mutant allele that we designate ngm2-1, which is defective with respect to induced mutagenesis. This mutant was isolated by screening mutagenized clones for reduced frequencies of reversion of the his1-7 allele, induced by N-methyl-N-nitro-N-nitrosoguanidine. As judged by the reversion of his1-7 and ilv1-92, ngm2-1 mutant strains are also deficient with respect to mutability induced by methyl methane sulfonate, ethyl methane sulfonate and, at least partially, by UV. UV-induced reversion of the ochre mutation arg4-17 and the frameshift mutation his4-38 was not much affected by ngm2-1, however. Like rev3 and rev7 mutations, ngm2-1 also has little influence on the reversion of the proline missense allele, cyc1-115. Ngm2-1 mutants are only at best very slightly more sensitive to the toxicity of the four mutagens used, and homozygous diploids sporulate normally.

  • References18
  • Citations10

Citations

Mentioned in this Paper

Methylnitronitrosoguanidine
HTN1 gene
Saccharomyces cerevisiae allergenic extract
Methanesulfonate
ARG8
ARG1
Proline
REV3L gene
REV3
HIS6

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