The isolation and characterization of two Stenotrophomonas maltophilia bacteriophages capable of cross-taxonomic order infectivity

BMC Genomics
Danielle L PetersJonathan J Dennis

Abstract

A rapid worldwide increase in the number of human infections caused by the extremely antibiotic resistant bacterium Stenotrophomonas maltophilia is prompting alarm. One potential treatment solution to the current antibiotic resistance dilemma is "phage therapy", the clinical application of bacteriophages to selectively kill bacteria. Towards that end, phages DLP1 and DLP2 (vB_SmaS-DLP_1 and vB_SmaS-DLP_2, respectively) were isolated against S. maltophilia strain D1585. Host range analysis for each phage was conducted using 27 clinical S. maltophilia isolates and 11 Pseudomonas aeruginosa strains. Both phages exhibit unusually broad host ranges capable of infecting bacteria across taxonomic orders. Transmission electron microscopy of the phage DLP1 and DLP2 morphology reveals that they belong to the Siphoviridae family of bacteriophages. Restriction fragment length polymorphism analysis and complete genome sequencing and analysis indicates that phages DLP1 and DLP2 are closely related but different phages, sharing 96.7 % identity over 97.2 % of their genomes. These two phages are also related to P. aeruginosa phages vB_Pae-Kakheti_25 (PA25), PA73, and vB_PaeS_SCH_Ab26 (Ab26) and more distantly related to Burkholderia cepacia com...Continue Reading

References

Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Jan 5, 1999·Applied and Environmental Microbiology·V PellegrinG Cottenceau
May 30, 2002·Nucleic Acids Research·Arthur L DelcherSteven L Salzberg
Jul 24, 2003·Protein Science : a Publication of the Protein Society·Agnieszka S JunckerAnders Krogh
Aug 29, 2003·Nature·Matthew B SullivanSallie W Chisholm
Sep 25, 2004·Antimicrobial Agents and Chemotherapy·Steven HagensUdo Bläsi
Mar 5, 2005·Applied and Environmental Microbiology·Hsiao-Chuan ChangShu-Fen Weng
May 4, 2005·Trends in Microbiology·Lisa U MagnussonThomas Nyström
Jun 28, 2005·Nucleic Acids Research·Johannes SödingAndrei N Lupas
Sep 6, 2005·FEMS Microbiology Letters·Kimberley D Seed, Jonathan J Dennis
Nov 30, 2005·Comptes rendus biologies·Pascaline Lacroix-GueuMarie-Pierre Fontaine-Aupart
Jan 5, 2006·Molecular Microbiology·Miryam GrossGad Glaser
Jan 24, 2006·Journal of Bacteriology·Tony KwanJerry Pelletier
Nov 17, 2006·Environmental Microbiology·Martha R J Clokie, Nicholas H Mann
Jan 24, 2007·Bioinformatics·Arthur L DelcherSteven L Salzberg
Apr 19, 2007·Archives of Microbiology·Chiy-Rong ChenShu-Fen Weng
Sep 29, 2007·Journal of Molecular Biology·Elizabeth J SummerRy Young
Nov 22, 2007·Poultry Science·L BielkeB M Hargis
Feb 5, 2008·Applied and Environmental Microbiology·R BriandetM-P Fontaine-Aupart
Oct 28, 2008·Applied and Environmental Microbiology·Pilar GarcíaJuan E Suárez
Jun 23, 2009·Genome Research·Martin KrzywinskiMarco A Marra
Aug 8, 2009·Journal of Wound Care·D D RhoadsA Sulakvelidze
Aug 14, 2009·Clinical Otolaryngology : Official Journal of ENT-UK ; Official Journal of Netherlands Society for Oto-Rhino-Laryngology & Cervico-Facial Surgery·A WrightD R Harper
Dec 1, 2009·Plasmid·Monika MaciagAgnieszka Szalewska-Pałasz
Jan 1, 2009·Current Pharmaceutical Biotechnology·Elizabeth KutterStephen T Abedon
Aug 25, 2010·Protein Science : a Publication of the Protein Society·Joel BerryRy Young
Oct 27, 2010·BMC Genomics·Karlene H LynchJonathan J Dennis
Nov 27, 2010·Nucleic Acids Research·Aron Marchler-BauerStephen H Bryant
Sep 13, 2011·Expert Review of Anti-infective Therapy·Ben BurrowesMark C Enright
Jan 11, 2012·Clinical Microbiology Reviews·Joanna S Brooke
Feb 16, 2012·Bacteriophage·Stephen T AbedonElizabeth Martin Kutter
Apr 25, 2012·FEMS Immunology and Medical Microbiology·Elizabeth M RyanBrendan F Gilmore
May 9, 2012·Applied Microbiology and Biotechnology·Natia KarumidzeStephen C Hardies

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Citations

Jun 9, 2016·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Dóra DömötörTamás Kovács
Jun 18, 2017·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Arshan NasirGustavo Caetano-Anollés
Mar 3, 2018·Genome Announcements·Danielle L Peters, Jonathan J Dennis
Jan 31, 2018·Journal of Bacteriology·Taylor Miller-EnsmingerCatherine Putonti
Oct 14, 2017·The ISME Journal·Ksenia ArkhipovaLeonid A Kulakov
Aug 18, 2016·Nature·David Paez-EspinoNikos C Kyrpides
Feb 26, 2020·Infection and Immunity·Mihnea R Mangalea, Breck A Duerkop
Apr 18, 2019·BMC Genomics·Danielle L PetersJonathan J Dennis
Sep 5, 2020·International Journal of Molecular Sciences·Jaclyn G McCutcheonJonathan J Dennis
Feb 27, 2021·Nature Microbiology·Eric G SakowskiSarah P Preheim
Aug 11, 2021·Viruses·Philip Lauman, Jonathan J Dennis

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Datasets Mentioned

BETA
KR537872

Methods Mentioned

BETA
electron microscopy
transmission electron microscopy
PCR

Software Mentioned

Search
PHAST
Geneious
CD
HHpred
BLASTP
NUCmer
BLASTX
TMHMM
Excel

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