PMID: 6397235Dec 24, 1984Paper

The isolation and long-term culture of oligodendrocytes from newborn mouse brain

Brain Research
A SuzumuraD H Silberberg

Abstract

Using differential adhesion we successfully isolated relatively pure populations of mouse oligodendrocytes which can be maintained in vitro for more than two months. The highest percentage of galactocerebroside (GalC)-positive oligodendrocytes was 95% at 3 days after isolation. Thereafter, proliferation of astrocytes and fibroblasts occurred more quickly than did oligodendrocyte precursor division. GalC-positive oligodendrocytes rarely incorporate [3H]thymidine so that the use of a mitotic inhibitor (5 X 10(-6)M AraC) reduced the number of non-oligodendrocytes so as to maintain the purity of oligodendrocytes at more than 75% for 14 days in culture. This system will be of use for immunological and virological studies which require viable cultured mouse oligodendrocytes.

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Citations

Sep 1, 1993·Journal of Neuroscience Research·S Amur-UmarjeeA T Campagnoni
Nov 26, 1990·Brain Research·D KajikawaM Shimo-oku
Oct 1, 1993·Neuroscience Letters·M SawadaT Marunouchi
Jun 1, 1995·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·M F MehlerJ A Kessler
Jun 1, 1995·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·M SawadaT Marunouchi
Dec 24, 1997·Neuroscience Letters·F ImaiT Kanno
Sep 13, 1994·Proceedings of the National Academy of Sciences of the United States of America·S ByravanA T Campagnoni
Nov 5, 2004·Molecular Biology of the Cell·María G ThomasGraciela L Boccaccio
Mar 11, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Pablo M PaezAnthony T Campagnoni
May 7, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Pablo M PaezAnthony T Campagnoni
May 22, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Pablo M PaezAnthony T Campagnoni
Dec 1, 1990·Journal of Neuroimmunology·A SuzumuraT Marunouchi
Aug 1, 1988·Journal of Neuroimmunology·K Kawai, B Zweiman
Jun 1, 1988·Journal of Neuroimmunology·E LaviS R Weiss
Jul 6, 2006·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Fumihiro ImaiMakoto Sawada
Sep 4, 2012·Annals of Neurology·Hiroko NobutaJames A Waschek
Nov 13, 2009·Journal of Neuroscience Research·Makoto HoriuchiTakayuki Itoh
Feb 20, 1989·Brain Research·A Suzumura, D H Silberberg
Mar 4, 2014·Journal of Neuroscience Methods·Yoshiaki NakayamaAkira Kurosaka
May 11, 2002·The Tohoku Journal of Experimental Medicine·Minka YoshikawaTetsuya Takayanagi

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