PMID: 5911526Mar 1, 1966

The isolation and partial characterization of glycolipids of normal human leucocytes

The Biochemical Journal
C J MirasG M Levis

Abstract

1. The lipids of purified human leucocytes were extracted with chloroform-methanol and the extract was washed with water. Glycolipids, isolated by Florisil chromatography, were subjected to mild alkaline hydrolysis and the alkali-resistant fraction was fractionated on a silicic acid column. 2. Three classes of glycolipid were separated. The less polar, containing 3.6% of the total glycolipid hexose as galactose, was tentatively identified as ceramide monohexoside. The major glycolipid fraction was characterized as ceramide dihexosides. The more polar glycolipids comprised 1.6% of the total glycolipid hexose as galactose and glucose (in the molar ratio 2:1) and were non-acidic. This class was separated as a mixture containing ninhydrin-positive glycolipids. 3. The ceramide dihexosides taken from two leucocyte preparations accounted for 15.2% and 16.4% by weight of the total lipids. 4. The carbohydrate moiety of the ceramide dihexosides contained galactose and glucose in the molar ratio 2:1. Partial acid hydrolysis and paper chromatography indicated that the hexoses are present as disaccharides, lactose being identified as one of them. 5. Palmitic acid (C(16:0)) and nervonic acid (C(24:1)) were the major fatty acids of this glyco...Continue Reading

Citations

Jan 1, 1982·Acta Paediatrica Scandinavica. Supplement·B Friis-Hansen
Jan 1, 1983·Acta Paediatrica Scandinavica. Supplement·B Friis-Hansen
Jan 1, 1970·The Journal of Pediatrics·C L WitzlebenO M Mohabbat
Aug 23, 1967·Biochemical and Biophysical Research Communications·G Hauser
May 10, 1968·Biochemical and Biophysical Research Communications·P P Dukes
Sep 1, 1974·Lipids·G M Levis, M Kesse-Elias
Oct 1, 1974·Lipids·J N Karli, G M Levis
Nov 1, 1973·Journal of Neurochemistry·J D MantzosG M Levis
Aug 1, 1980·European Journal of Biochemistry·C Vakirtzi-LemoniasG M Levis
Jun 9, 2009·American Journal of Physiology. Heart and Circulatory Physiology·Lusha Xiang, Robert L Hester
Aug 22, 1968·The New England Journal of Medicine·J N KanferS M Wolff
Apr 8, 1971·The New England Journal of Medicine·R J DesnickW Krivit
Jul 15, 1969·Klinische Wochenschrift·M Albrecht

Related Concepts

Paper Chromatography (Procedure)
Thin Layer Chromatography
Saturated Fat
Glycolipids
Hexoses
Leukocytes

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