PMID: 1201277Dec 26, 1975Paper

The isolation and structure of the core oligosaccharide sequences of IgM

Biochemistry
A L TarentinoF Maley

Abstract

Methods are presented for separating the three IgM heavy chain sialoglycopeptides associated with asparagines 170, 332, and 395. The core glycopeptide units containing the disaccharide fucosyl-N-acetylglucosamine were obtained through the use of an endo-beta-N-acetylglucosamindase from Diplococcus pneumoniae, following exoglycosidase treatment of the sialoglycopeptides. In addition to the core glycopeptides, high yields of a tetrasaccharide, (Man)3GlcNAc, were obtained. The fucose in the core disaccharide is glycosidically linked to the 6-O position of the N-acetylglucosamine residue in Asn-GlcNAc. This core unit is resistant to glycosyl asparaginase, but becomes susceptible to hydrolysis on removal of the fucosyl residue by a purified hen oviduct alpha-L-fucosidase. The core sequence of the immunoglobulin M sialoglycopeptides appears to be similar to that of most other asparagine-linked oligosaccharides in consisting of a basic unit composed of beta-D-Man-(1 leads to 4)beta-D-GlcNAc(1 leads to 4)beta-D-GlcNAc(1 leads to 4), but with L fucose linked alpha-(1 leads to 6) to the proximal GlcNAc. The two nonreducing terminal ends of (Man)3GlcNAc are linked to beta-D-Man by alpha-(1 leads to 3) and alpha(1 leads to 6) bonds, respec...Continue Reading

Citations

Apr 15, 1981·Archives of Biochemistry and Biophysics·J P AubertM H Loucheux-Lefebvre
Apr 29, 1983·Journal of Immunological Methods·A H Blair, T I Ghose
Sep 1, 1978·Immunochemistry·J L Winkelhake
Jun 19, 1993·Biochimica Et Biophysica Acta·J RühlmannE Köttgen
Aug 1, 1983·The Journal of Cell Biology·L Pollack, P H Atkinson
Dec 13, 2006·Journal of Molecular Biology·Yeming Wang, Hwai-Chen Guo
Aug 1, 1989·Archives of Biochemistry and Biophysics·N N AronsonM J Kuranda
Jan 1, 1977·Analytical Biochemistry·A L Tarentino, F Maley
Nov 26, 1999·Biochimica Et Biophysica Acta·N N Aronson
Jan 14, 2005·Glycobiology·Bryan Winchester

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