PMID: 37916Jun 21, 1979

The isolation of acyl-CoA derivatives as products of partial reactions in the microsomal chain elongation of fatty acids

Biochimica Et Biophysica Acta
J T Bernert, H Sprecher

Abstract

An analysis of overall chain elongation, condensation, beta-hydroxyacyl-CoA dehydrase and 2-trans enoyl-CoA reductase reactions, using the appropriate CoA derivatives as substrates which are required in the microsomal chain elongation of both palmitoyl-CoA and 6,9-octadecadienoyl-CoA, demonstrated that in each instance, the products of these reactions were the CoA derivatives. Reverse dehydrase reactions run with 2-trans enoyl-CoA derivatives as substrates, in the absence of NADPH, revealed that the product was the beta-hydroxyacyl-Coa. In the presence of NADPH, incubations with beta-hydroxyacyl-CoA demonstrated that both the 2-trans derivatives and the alpha, beta-saturated product were recovered as their CoA derivatives. These latter findings are more consistent with the involvement of discrete dehydrase and 2-trans-enoyl-CoA reductase enzymes rather than a single protein catalyzing two reactions.

References

Jul 1, 1968·Archives of Biochemistry and Biophysics·D N BurtonJ W Porter
Feb 1, 1956·The Journal of Clinical Investigation·V P DOLE
Nov 27, 2009·Interactive Cardiovascular and Thoracic Surgery·Omar A ArajiJosé M Barquero

Citations

Jun 1, 1989·Mechanisms of Ageing and Development·S Laganiere, B P Yu
Jul 27, 2004·Archives of Insect Biochemistry and Physiology·M Patricia Juárez
Feb 15, 1985·Archives of Biochemistry and Biophysics·G J Anderson, P E Kolattukudy
Feb 1, 1995·Prostaglandins, Leukotrienes, and Essential Fatty Acids·H SprecherB S Mohammed
Aug 24, 1999·Current Opinion in Clinical Nutrition and Metabolic Care·H Sprecher
May 21, 2020·The Plant Journal : for Cell and Molecular Biology·Lin ChenGuoxin Shen
Jul 5, 1996·The Journal of Biological Chemistry·D L LuthriaH Sprecher

Related Concepts

Acyl Coenzyme A
Metazoa
Saturated Fat
Hydroxylation
Microsomes, Liver
NADP
Palmitoyl Coenzyme A
Rats, Laboratory
In Vitro [Publication Type]

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