PMID: 6162155Dec 20, 1980Paper

The isolation of cloned cDNA sequences which are differentially expressed in human lymphocytes and fibroblasts

Nucleic Acids Research
J CramptonR Williamson

Abstract

Poly(A)+ RNA populations derived from normal lymphocytes and fibroblasts have been compared by hybridising each RNA to cDNA derived from the other RNA population. This indicated that approximately 75% of the sequences were common to both, and that these were present at different concentrations in the two cell types. The two RNA populations were further compared by hybridising them to a cDNA recombinant library derived from lymphocyte poly(A)+ RNA. This allowed the identification of clones containing sequences which are abundant in lymphocyte poly(A)+ RNA but absent or rare in fibroblast poly(A)+ RNA. A direct estimation of the abundance of five of these sequences in lymphocyte cDNA demonstrated that clones can be detected by such a procedure if they represent 0.2% or greater of the original cDNA population.

References

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Citations

Jan 1, 1992·Mammalian Genome : Official Journal of the International Mammalian Genome Society·T M GressH Lehrach
Jun 21, 2006·Nature Immunology·Gordon HyattChristophe Benoist
Jul 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·G J FreemanH Cantor
Jul 1, 1983·The Journal of Experimental Medicine·M KronenbergL Hood
Jan 1, 1997·Annual Review of Biochemistry·C G SagerströmH L Sive
Apr 1, 1992·International Journal of Cancer. Journal International Du Cancer·M G DenisK Meflah
Nov 6, 1989·European Journal of Biochemistry·K Gale, J Crampton
Oct 1, 1984·Immunological Reviews·M M DavisS M Hedrick
Jan 1, 1987·Roux's Archives of Developmental Biology : the Official Organ of the EDBO·Wolfgang MeyerhofWalter Knöchel
Jan 1, 2015·Microarrays·Kenichi InaokaShuji Nomoto

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