PMID: 5914246Sep 1, 1966

The isolation of hyaluronic acid and chondroitin sulphate from kidneys and their reaction with urinary hyaluronidase

The Journal of Physiology
S E Dicker, C S Franklin

Abstract

1. Extracts of the cortex and the medulla of fresh kidneys from pigs, sheep and dogs were analysed for the presence of acid mucopolysaccharides.2. Acid mucopolysaccharides were found in the kidneys of the three species and were evenly distributed between cortex and medulla.3. The acid mucopolysaccharides isolated from either the medulla or the cortex of kidneys contained equal amounts of sulphated and nonsulphated fractions; these could be identified as chondroitin sulphate and hyaluronic acid, respectively.4. Urinary hyaluronidase was isolated from urine and estimated by means of turbidity measurements.5. Urine hyaluronidase depolymerizes the acid mucopolysaccharides extracted from either the cortex or the medulla of the kidneys from pig, sheep and dog.6. The enzymic activity of urine hyaluronidase could be expressed as units/mg of protein. The optimum pH activity of the enzyme is at about 4.0.

Citations

Jan 1, 1967·Zeitschrift für Zellforschung und mikroskopische Anatomie·F R Johnson, S J Darnton
Jan 1, 1985·CRC Critical Reviews in Toxicology·P H Bach, J W Bridges
Jan 1, 1985·Critical Reviews in Toxicology·P H Bach, J W Bridges
Dec 1, 1992·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·H YamaguchiM Morisada
Aug 31, 2016·Matrix Biology : Journal of the International Society for Matrix Biology·Astgik PetrosyanLaura Perin
Nov 1, 1973·Quarterly Reviews of Biophysics·D J Cunningham

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