The isolation of soyasaponins by fractional precipitation, solid phase extraction, and low pressure liquid chromatography

International Journal of Food Sciences and Nutrition
D M GurfinkelA V Rao

Abstract

Bioactive soyasaponins are present in soybean (Glycine max). In this study, the isolation of soyasaponins in relatively pure form (>80%) using precipitation, solid phase extraction and reverse phase low pressure liquid chromatography (RP-LPLC) is described. Soy flour soyasaponins were separated from non-saponins by methanol extraction and precipitation with ammonium sulphate. Acetylated group A soyasaponins were isolated first by solid phase extraction followed by RP-LPLC (solvent: ethanol-water). Soyasaponins, from a commercial preparation, were saponified and fractionated into deacetylated group A and group B soyasaponins by solid phase extraction (methanol-water). Partial hydrolysis of group B soyasaponins produced a mixture of soyasaponin III and soyasapogenol B monoglucuronide. RP-LPLC of deacetylated group A soyasaponins separated soyasaponin A1 and A2 (38% methanol); of group B soyasaponins isolated soyasaponin I (50% ethanol); and of the partial hydrolysate separated soyasaponin III from soyasapogenol B monoglucuronide (50% ethanol). This methodology provides soyasaponin fractions that are suitable for biological evaluation.

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Citations

Aug 25, 2009·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Wei Zhang, David G Popovich
Jun 13, 2009·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Wei ZhangDavid G Popovich
Mar 21, 2007·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Jun-Xia XiaoSheng-Hua Zhang
May 24, 2008·Natural Product Reports·Otto Sticher
Sep 25, 2012·Journal of Separation Science·Dayun ZhaoXiangjun Sun
Jan 1, 2014·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Hajime KatanoMasakazu Takahashi
Mar 26, 2008·Journal of Agricultural and Food Chemistry·Wei Zhang, David G Popovich
Mar 30, 2010·Journal of Agricultural and Food Chemistry·Wei Zhang, David G Popovich
Apr 3, 2009·Journal of Agricultural and Food Chemistry·Wei ZhangDavid G Popovich

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