The itinerary of autophagosomes: from peripheral formation to kiss-and-run fusion with lysosomes.

Traffic
Luca JahreissDavid C Rubinsztein

Abstract

Macroautophagy, a constitutive process in higher eukaryotic cells, mediates degradation of many long-lived proteins and organelles. The actual events occurring during the process in the dynamic system of a living cell have never been thoroughly investigated. We aimed to develop a live-cell assay in which to follow the complete itinerary of an autophagosome. Our experiments show that autophagosomes are formed randomly in peripheral regions of the cell. They then move bidirectionally along microtubules, accumulating at the microtubule-organizing centre, in a similar way to lysosomes. Their centripetal movement is dependent on the motor protein dynein and is important for their fusion with lysosomes. Initially, autophagosomes dock on to lysosomes, independent of lysosomal acidification. Two kinds of fusion then occur: complete fusions, creating a hybrid organelle, or more often kiss-and-run fusions, i.e. transfer of some content while still maintaining two separate vesicles. Surprisingly, the autophagolysosomal compartment seems to be more long lived than expected. Our study documents many aspects of autophagosome behaviour, adding to our understanding of the mechanism and control of autophagy. Indeed, although the formation of au...Continue Reading

References

Jul 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·C M Schworer, G E Mortimore
Feb 29, 1988·Biochemical and Biophysical Research Communications·P B Gordon, P O Seglen
Sep 1, 1987·The Journal of Cell Biology·R Matteoni, T E Kreis
Nov 1, 1984·Journal of Neurochemistry·P Ekström, M Kanje
Mar 28, 1998·The Journal of Biological Chemistry·T Noda, Y Ohsumi
Mar 27, 2001·The Journal of Cell Biology·N MizushimaT Yoshimori
Jul 16, 2003·Molecular and Cellular Biology·Vadim AtlashkinGabriele Fischer von Mollard
May 13, 2004·Journal of Cell Science·Maximiliano G GutierrezMaría I Colombo
Jul 10, 2004·Current Biology : CB·Michael A Welte
Sep 2, 2004·Journal of Cell Science·Stefanie JägerEeva-Liisa Eskelinen
Feb 23, 2005·Current Biology : CB·Nicholas A BrightJ Paul Luzio
Apr 29, 2005·The Journal of Biological Chemistry·Takahide KounoKeiichi Kawano
Jun 28, 2005·Nature Genetics·Brinda RavikumarDavid C Rubinsztein
Jul 29, 2006·Autophagy·Tamotsu Yoshimori
Mar 31, 2007·Nature Reviews. Drug Discovery·David C RubinszteinDaniel J Klionsky
Nov 17, 2007·Genes & Development·Noboru Mizushima

❮ Previous
Next ❯

Citations

Jun 30, 2012·Acta Neuropathologica·Darius Ebrahimi-FakhariPamela J McLean
Jan 28, 2009·Apoptosis : an International Journal on Programmed Cell Death·Avital Eisenberg-Lerner, Adi Kimchi
Jun 6, 2013·Neurochemical Research·Marzena Ułamek-KoziołRyszard Pluta
May 21, 2013·Cell Biochemistry and Biophysics·Veronika SeiberlichChristiane Richter-Landsberg
Jul 23, 2013·The International Journal of Biochemistry & Cell Biology·Bahram KasmapourMaximiliano Gabriel Gutierrez
Apr 11, 2013·Chemical Reviews·Chad P SatoriEdgar A Arriaga
Feb 5, 2013·Acta Pharmacologica Sinica·Warren E HochfeldDavid C Rubinsztein
Aug 29, 2009·Cell Death and Differentiation·S Luo, D C Rubinsztein
Nov 23, 2012·Cell Death & Disease·M RacitiL Di Renzo
Jun 16, 2010·Cell Research·Maryam MehrpourPatrice Codogno
Sep 14, 2011·Cell Research·Ellen WirawanPeter Vandenabeele
Mar 12, 2011·Nature Cell Biology·Viktor I KorolchukDavid C Rubinsztein
Sep 1, 2012·Nature Reviews. Drug Discovery·David C RubinszteinBeth Levine
Feb 6, 2010·Proceedings of the National Academy of Sciences of the United States of America·Carol S LeungGraham S Taylor
Nov 28, 2012·Proceedings of the National Academy of Sciences of the United States of America·Sue E CrawfordMary K Estes
May 21, 2010·The Journal of Biological Chemistry·Camille GeeraertChristian Poüs
Feb 12, 2010·The Journal of Biological Chemistry·Maurizio RennaDavid C Rubinsztein
Aug 21, 2012·Carcinogenesis·Lisa B Frankel, Anders H Lund
Aug 9, 2012·Human Molecular Genetics·Matthew J ElrickAndrew P Lieberman
Mar 4, 2011·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Yuan TianPaul Greengard
Jun 6, 2009·Neuropathology : Official Journal of the Japanese Society of Neuropathology·Midori YamamotoMasaru Himeno
Aug 8, 2009·Biological Reviews of the Cambridge Philosophical Society·Iryna MonastyrskaFulvio Reggiori
Oct 15, 2011·Pigment Cell & Melanoma Research·Agnieszka Checinska, María S Soengas
Apr 17, 2009·Journal of Virology·Matthew P TaylorWilliam T Jackson
Aug 6, 2009·Annual Review of Genetics·Congcong He, Daniel J Klionsky
Apr 8, 2009·Molecular Neurodegeneration·Philipp A Jaeger, Tony Wyss-Coray
May 23, 2009·Journal of Cell Science·Brinda RavikumarDavid C Rubinsztein
Jan 19, 2011·Journal of Cell Science·Maurizio RennaDavid C Rubinsztein
Apr 5, 2008·Cell Structure and Function·Shunsuke KimuraTamotsu Yoshimori
Mar 31, 2010·PloS One·Kimberly R DrakeAnne K Kenworthy
Jun 19, 2010·PloS One·Philipp A JaegerTony Wyss-Coray
Oct 14, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Darius Ebrahimi-FakhariVivek K Unni

❮ Previous
Next ❯

Methods Mentioned

BETA
electron microscopy

Software Mentioned

SPSS
graphpad prism
zeiss lsm image browser
ms excel

Related Concepts

Related Feeds

Autophagy & Model Organisms

Autophagy is a cellular process that allows degradation by the lysosome of cytoplasmic components such as proteins or organelles. Here is the latest research on autophagy & model organisms