The K1 capsular polysaccharide of Acinetobacter baumannii strain 307-0294 is a major virulence factor.

Infection and Immunity
Thomas A RussoAnthony A Campagnari

Abstract

Acinetobacter baumannii is a pathogen of increasing medical importance with a propensity to be multidrug resistant, thereby making treatment challenging. Little is known of virulence traits in A. baumannii. To identify virulence factors and potential drug targets, random transposon (Tn) mutants derived from the A. baumannii strain AB307-0294 were screened to identify genes essential for growth in human ascites fluid in vitro, an inflammatory exudative fluid. These studies led to the identification of two genes that were predicted to be required for capsule polymerization and assembly. The first, ptk, encodes a putative protein tyrosine kinase (PTK), and the second, epsA, encodes a putative polysaccharide export outer membrane protein (EpsA). Monoclonal antibodies used in flow cytometric and Western analyses confirmed that these genes are required for a capsule-positive phenotype. A capsule-positive phenotype significantly optimized growth in human ascites fluid, survival in human serum, and survival in a rat soft tissue infection model. Importantly, the clearance of the capsule-minus mutants AB307.30 (ptk mutant, capsule minus) and AB307.45 (epsA mutant, capsule minus) was complete and durable. These data demonstrated that the ...Continue Reading

References

Jan 1, 1992·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·N M AnsteyK M Withnall
Jun 28, 1996·Journal of Molecular Biology·B DuclosA J Cozzone
Apr 1, 1996·Clinical Microbiology Reviews·E Bergogne-Bérézin, K J Towner
May 3, 2003·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·Maria Virginia Villegas, Alan I Hartstein
Jul 29, 2004·The Annals of Pharmacotherapy·Rupali Jain, Larry H Danziger
Aug 11, 2004·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·M Van LooverenUNKNOWN ARPAC Steering Group
Aug 25, 2004·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Maria Cristina Bronharo TognimHélio S Sader
Aug 17, 2005·Emerging Infectious Diseases·Kepler A DavisPaula J Gray
Aug 19, 2005·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Robert GaynesUNKNOWN National Nosocomial Infections Surveillance System
Oct 12, 2005·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·M-L Joly-Guillou
Nov 26, 2005·The Journal of Hospital Infection·Z JerassyD Raveh
Dec 2, 2005·Emerging Infectious Diseases·Christian GarzoniJorge Garbino
Feb 1, 2006·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Pierre Edouard Fournier, Hervé Richet
Jun 30, 2006·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·Hervé Richet, Pierre Edouard Fournier
Oct 10, 2006·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Effrossyni Gkrania-Klotsas, Ronald C Hershow
Feb 7, 2007·Proceedings of the National Academy of Sciences of the United States of America·Richard F CollinsJames H Naismith
Mar 3, 2007·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·M E Falagas, E A Karveli
May 23, 2007·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Paul ScottBruno Petruccelli
Jul 20, 2007·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Erica N JohnsonClinton K Murray
Nov 21, 2007·Journal of Bacteriology·Thomas W LoehfelmAnthony A Campagnari
Jun 11, 2008·Infection and Immunity·Thomas A RussoAnthony A Campagnari
Jul 16, 2008·Clinical Microbiology Reviews·Anton Y PelegDavid L Paterson
Aug 23, 2008·Science·David A RaskoVanessa Sperandio
Sep 20, 2008·Science·David J Payne
Oct 22, 2008·Journal of Bacteriology·Mark D AdamsSteven R Gill
Nov 28, 2008·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Helen W BoucherJohn Bartlett
Jan 16, 2009·The Journal of Infectious Diseases·Thomas A RussoTimothy C Umland

❮ Previous
Next ❯

Citations

Jul 4, 2012·The Journal of Microbiology·Christin N McQuearyDaniel V Zurawski
Sep 13, 2012·Journal of Proteome Research·Jose Antonio MendezGermán Bou
Jan 11, 2013·The Journal of Infectious Diseases·Lauren B KingLarry S McDaniel
May 22, 2013·Antimicrobial Agents and Chemotherapy·Greg HarrisWangxue Chen
May 8, 2013·Infection and Immunity·Bart A EijkelkampMelissa H Brown
Nov 16, 2011·Infection and Immunity·Kari A Brossard, Anthony A Campagnari
Jul 25, 2012·Infection and Immunity·Leticia V BentancorTomás Maira-Litrán
May 23, 2012·Journal of Bacteriology·Leticia V BentancorTomás Maira-Litrán
Feb 24, 2012·Canadian Journal of Microbiology·Marie KempfMarie-Laure Joly-Guillou
Oct 12, 2011·BMC Microbiology·Pier Paolo Di NoceraRaffaele Zarrilli
Apr 20, 2011·Microbes and Environments·Hamuel James DoughariSpinney Benade
Aug 11, 2011·PloS One·Luísa C S AntunesPaolo Visca
Mar 26, 2013·PloS One·Daniel N FarrugiaIan T Paulsen
Mar 26, 2013·PloS One·Michael D CarruthersRobert S Munson
Mar 29, 2013·Future Microbiology·Biao YuanMingshu Wang
Dec 4, 2013·Antioxidants & Redox Signaling·Alistair J Standish, Renato Morona
Apr 2, 2014·Glycobiology·Johanna J KenyonCristina De Castro
Nov 19, 2014·Future Microbiology·Timothy C UmlandThomas A Russo
Nov 26, 2014·BMC Genomics·Bart A EijkelkampMelissa H Brown
Jul 15, 2015·Human Vaccines & Immunotherapeutics·Wangxue Chen
Oct 13, 2011·IUBMB Life·Gustavo M Cerqueira, Anton Y Peleg
May 30, 2012·Cellular Microbiology·Brittany L Mortensen, Eric P Skaar
Jun 21, 2013·Molecular Microbiology·Robert G Lees-MillerMario F Feldman
Jul 24, 2014·Molecular Oral Microbiology·A M RichardsR J Lamont
Dec 24, 2011·Journal of Medical Microbiology·Matthew E WandJ Mark Sutton

❮ Previous
Next ❯

Related Concepts

Related Feeds

Acinetobacter Infections

Acinetobacter infections have become common in hospitalized patients, especially in the intensive care unit setting and are difficult to treat due to their propensity to develop antimicrobial drug resistance. Discover the latest research on Acinetobacter Infections here.