PMID: 7539141May 23, 1995Paper

The kappa-opioid receptor is primarily postsynaptic: combined immunohistochemical localization of the receptor and endogenous opioids

Proceedings of the National Academy of Sciences of the United States of America
U ArvidssonMartin W Wessendorf

Abstract

Antisera were raised against a synthetic peptide corresponding to the carboxyl terminus of the kappa-opioid receptor (KOR1). Specificity of the antisera was verified by staining of COS-7 cells transfected with KOR1 and epitope-tagged KOR1 cDNAs, by recognition by the antisera of proteins on Western blots of both transfected cells and brain tissue, by the absence of staining of both brain tissue and transfected cells after preabsorption of the antisera with the cognate peptide, and on the strong correlation between the distribution of KOR1 immunoreactivity and that of earlier ligand binding and in situ hybridization studies. Results indicate that KOR1 in neurons is targeted into both the axonal and somatodendritic compartments, but the majority of immunostaining was seen in the somatodendritic compartment. In sections from rat and guinea pig brain, prominent KOR1 staining was seen in the ventral forebrain, hypothalamus, thalamus, posterior pituitary, and midbrain. While the staining pattern was similar in both species, distinct differences were also observed. The distribution of preprodynorphin and KOR1 immunoreactivity was complementary in many brain regions, suggesting that KOR1 is poised to mediate the physiological actions o...Continue Reading

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Citations

Feb 10, 2004·Brain Research. Developmental Brain Research·Eli M MrkusichDarren J Day
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Mar 16, 2012·Reproductive Biomedicine Online·Ekaitz AgirregoitiaNaiara Agirregoitia
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Mar 31, 2007·Psychiatry Research·Marco Bortolato, Marylou V Solbrig

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