PMID: 9555886Apr 29, 1998Paper

The katX gene, which codes for the catalase in spores of Bacillus subtilis, is a forespore-specific gene controlled by sigmaF, and KatX is essential for hydrogen peroxide resistance of the germinating spore

Journal of Bacteriology
I BagyanP Setlow

Abstract

Previous work has shown that the katX gene encodes the major catalase in dormant spores of Bacillus subtilis but that this enzyme has no role in dormant spore resistance to hydrogen peroxide. Expression of a katX-lacZ fusion began at approximately h 2 of sporulation, and >75% of the katX-driven beta-galactosidase was packaged into the mature spore. A mutation in the gene coding for the sporulation-specific RNA polymerase sigma factor sigmaF abolished katX-lacZ expression, while mutations in genes encoding sigmaE, sigmaG, and sigmaK did not. Induction of sigmaF synthesis in vegetative cells also resulted in katX-lacZ expression, while induction of sigmaG expression did not; the katX-lacZ fusion was also not induced by hydrogen peroxide. Upstream of the in vivo katX transcription start site there are sequences with good homology to those upstream of known sigmaF-dependent start sites. These data indicate that katX is an additional member of the forespore-specific sigmaF regulon. A mutant in the katA gene, encoding the major catalase in growing cells, was sensitive to hydrogen peroxide during sporulation, while a katX mutant was not. However, outgrowth of katX spores, but not katA spores, was sensitive to hydrogen peroxide. Conseq...Continue Reading

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Citations

Jun 10, 2004·Microbiology and Molecular Biology Reviews : MMBR·David W Hilbert, Patrick J Piggot
Feb 24, 2015·Annual Review of Food Science and Technology·Aleksandra ChecinskaMalcolm Burbank
Jan 25, 2011·Journal of Bacteriology·Valéria C S ItalianiMarilis V Marques
Sep 7, 2000·Microbiology and Molecular Biology Reviews : MMBR·W L NicholsonP Setlow
Aug 13, 2003·Eukaryotic Cell·Shaday MichánWilhelm Hansberg
Dec 24, 2011·Journal of Bacteriology·Melinda J FaulknerJohn D Helmann
Jul 7, 2009·Annual Review of Microbiology·Peter Zuber
Sep 30, 2016·MicrobiologyOpen·Joelie V ZwickMelinda J Faulkner
Jul 29, 2000·FEMS Microbiology Letters·L Casillas-MartinezP Setlow
Jan 24, 2018·Nucleic Acids Research·Indra BervoetsDaniel Charlier
Nov 18, 2020·Nature Communications·Maarten Van BremptMarjan De Mey
Oct 6, 1998·Applied and Environmental Microbiology·B Setlow, P Setlow
Dec 1, 2001·Free Radical Biology & Medicine·A DíazW Hansberg
Jun 19, 2004·Journal of Bacteriology·Karen CarniolRichard Losick
Mar 6, 1999·Microbiology and Molecular Biology Reviews : MMBR·A Driks
May 24, 2006·Journal of Bioscience and Bioengineering·Issei KobayashiKenji Inagaki

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