The kidney expression of matrix metalloproteinase-9 in the diabetic nephropathy of Kkay mice

Journal of Diabetes and Its Complications
Guo Qing-HuaMu Yi-Ming

Abstract

Disorder of the extracellular matrix has been suggested to play a role in the development of diabetic nephropathy. The aim of the present study was to examine the expression of matrix metalloproteinase-9 (MMP-9) in the kidney of Kkay mice, a type 2 diabetic model. Eight Kkay control mice (KA) and eight Kkay diabetic mice (KB) were fed with the same diets for 4 months and sacrificed after glucose levels and weight were measured. The renal pathological changes were examined by periodic acid-Schiff and periodic acid-silver methenamine staining, and the glomerulosclerosis indexes (GIs) were calculated. The levels of MMP-9 protein in the kidney tissue were assessed by immunohistochemistry. The gene expression of MMP-9 was measured by reverse transcription polymerase chain reaction. The diabetic group had morphological findings of diabetic nephropathy, and their GI (240.0+/-17.3) was much higher than that of the control group (118.4+/-15.1) (P<.01). The mean positive area of MMP-9 protein in the glomeruli of Kkay mice in the KB group was higher than that in the KA group. The expression of MMP-9 messenger RNA level in the diabetic KB group was up-regulated when compared with the KA group. The expression of MMP-9 in the kidney of mice ...Continue Reading

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Citations

Oct 21, 2010·Endocrine·Kathryn M ThrailkillJohn L Fowlkes
May 1, 2009·Diabetes/metabolism Research and Reviews
Jan 13, 2009·Biochimica Et Biophysica Acta·Jarbas S Roriz-FilhoMatheus Roriz-Cruz
Feb 13, 2020·Journal of Clinical Medicine·Nuria Garcia-FernandezMaría José Soler
Feb 10, 2017·Pharmacological Reports : PR·George J Dugbartey

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