The kinesin KIF14 is overexpressed in medulloblastoma and downregulation of KIF14 suppressed tumor proliferation and induced apoptosis

Laboratory Investigation; a Journal of Technical Methods and Pathology
Kay Ka-Wai LiHo-Keung Ng

Abstract

Medulloblastoma (MB) is the most common malignant brain tumor in childhood. At present, there is no well-established targeted drug for majority of patients. The kinesin family member 14 (KIF14) is a novel oncogene located on chromosome 1q and is dysregulated in multiple cancers. The objectives of this study were to evaluate KIF14 expression and chromosome 1q copy number in MB, and to delineate its biological functions in MB pathogenesis. By quantitative RT-PCR and immunohistochemistry, we found KIF14 was overexpressed in MB. Increased KIF14 expression at protein level was strongly associated with shorter progression-free survival (P=0.0063) and overall survival (P=0.0083). Fluorescence in situ hybridization (FISH) analysis confirmed genomic gain of chromosome 1q in 17/93 (18.3%) of MB. Combined genetic and immunohistochemical analyses revealed that 76.5% of MB with 1q gain showed consistent overexpression of KIF14, and a tight link between chromosome 1q gain and KIF14 overexpression (P=0.03). Transient, siRNAs-mediated downregulation of KIF14 suppressed cell proliferation and induced apoptosis in two MB cell lines. Stably KIF14 knockdown by shRNAs inhibited cell viability, colony formation, migration and invasion, and tumor sph...Continue Reading

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Citations

Mar 13, 2019·Brain Pathology·Zhi-Feng ShiHo-Keung Ng
May 1, 2019·International Journal of Molecular Sciences·Gianmarco PallaviciniFerdinando Di Cunto
Nov 6, 2018·Scientific Reports·Kamakshi SishtlaTimothy W Corson
Jul 6, 2020·Cancer Biomarkers : Section a of Disease Markers·Zeling CaiHanyang Liu
Nov 3, 2020·Frontiers in Molecular Biosciences·Xiaowei ChenXiaobing Shen
Feb 14, 2021·Journal of Nanobiotechnology·Karmveer YadavGautam Kaul
Jul 3, 2021·Cancers·Anna Klimaszewska-WiśniewskaMaciej Gagat

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