The kinesin-like protein Pavarotti functions noncanonically to regulate actin dynamics.

The Journal of Cell Biology
Mitsutoshi NakamuraSusan M Parkhurst

Abstract

Pavarotti, the Drosophila MKLP1 orthologue, is a kinesin-like protein that works with Tumbleweed (MgcRacGAP) as the centralspindlin complex. This complex is essential for cytokinesis, where it helps to organize the contractile actomyosin ring at the equator of dividing cells by activating the RhoGEF Pebble. Actomyosin rings also function as the driving force during cell wound repair. We previously showed that Tumbleweed and Pebble are required for the cell wound repair process. Here, we show that Pavarotti also functions during wound repair and confirm that while Pavarotti, Tumbleweed, and Pebble are all used during this cellular repair, each has a unique localization pattern and knockdown phenotype, demonstrating centralspindlin-independent functions. Surprisingly, we find that the classically microtubule-associated Pavarotti binds directly to actin in vitro and in vivo and has a noncanonical role directly regulating actin dynamics. Finally, we demonstrate that this actin regulation by Pavarotti is not specific to cellular wound repair but is also used in normal development.

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Methods Mentioned

BETA
GTPase
nucleotide exchange
confocal microscopy
Fluorescence
GTPases
PCR
fluorescence microscopy
Assay

Software Mentioned

Airyscan
Pav DEAD
GLCM
Zen
R
MetaMorph
Prism
Fiji
Matlab

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